ERCC6L Antibody - Azide and BSA Free

Western Blot: ERCC6L Antibody [H00054821-D01P] - Analysis of ERCC6L expression in human colon.

Immunocytochemistry/Immunofluorescence: ERCC6L Antibody [H00054821-D01P] - IF analysis of ERCC6L in mouse and human cell lines. Image courtesy of product review submitted by Corentin Laulier.

Western Blot: ERCC6L Antibody [H00054821-D01P] - Analysis of ERCC6L expression in transfected 293T cell line by ERCC6L polyclonal antibody.Lane 1: ERCC6L transfected lysate(46.20 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ERCC6L Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit ERCC6L Antibody - Azide and BSA Free (H00054821-D01P) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-ERCC6L Antibody: Cited in 11 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: ERCC6L (AAH08808.1, 1 a.a. - 419 a.a.) full-length human protein. MEKSFATKNEAVQKETLQEGPKQEALQEDPLESFNYVLSKSTKADIGPNLDQLKDDEILRHCNPWPIISITNESQNAESNVSIIEIADDLSASHSALQDAQASEAKLEEEPSASSPQYACDFNLFLEDSADNRQNFSSQSLEHVEKENSLCGSAPNSRAGFVHSKTCLSWEFSEKDDEPEEVVVKAKIRSKARRIVSDGEDEDDSFKDTSSINPFNTSLFQFSSVKQFDASTPKNDISPPGRFFSSQIPSSVNKSMNSRRSLASRRSLINMVLDHVEDMEERLDDSSEAKGPEDYPEEGVEESSGEASKYTEEDPSGETLSSENKSSWLMTSKPSALAQETSLGAPEPLSGEQLVGSPQDKAAEATNDYETLVKRGKELKECGKIQEALNCLVKALDIKSADPEVMLLTLSLYKQLNNN
• Specificity: ERCC6L - excision repair cross-complementing rodent repair deficiency, complementation group 6-like,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ERCC6L
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. This antibody may also be useful in Immunofluorescence, as per a customer product review. Immunocytochemistry/Immunofluorescence was reported in scientific literature. Use in Immunohistochemistry reported in scientific literature (PMID 24589582)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ERCC6L Antibody - Azide and BSA Free

• ATP-dependent helicase ERCC6-like
• DNA excision repair protein ERCC-6-like
• EC 3.6.1
• EC 3.6.4.12
• excision repair cross-complementing rodent repair deficiency, complementationgroup 6-like
• excision repair protein ERCC6-like
• FLJ20105
• MGC131695
• PICHexcision repair cross-complementing rodent repair deficiency complementationgroup 6 - like
• PLK1-interacting checkpoint helicase
• SNF2/RAD54 family protein
• Tumor antigen BJ-HCC-15

Background

DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERCC6L Antibody (H00054821-D01P)(11)

Publications using H00054821-D01P

• Hamza M, Geraldine B, Gaelle F et al. Cytidine deaminase deficiency in tumor cells is associated with sensitivity to a naphthol derivative and a decrease in oncometabolite levels. Cell Mol Life Sci. 2022-08-04 [PMID: 35925417]
• Sandra S, Geraldine B, Rosine O et al. A decrease in NAMPT activity impairs basal PARP-1 activity in cytidine deaminase deficient-cells, independently of NAD+. Sci Rep. 2020-08-17 [PMID: 32807821]
• Yokoyama H, Moreno-Andres D, Astrinidis SA et al. Chromosome alignment maintenance requires the MAP RECQL4, mutated in the Rothmund-Thomson syndrome. Life Sci Alliance. 2019-02-04 [PMID: 30718377]
• Tiwari A, Addis Jones O, Chan KL. et al. 53BP1 can limit sister-chromatid rupture and rearrangements driven by a distinct ultrafine DNA bridging-breakage process. Nat Commun 2018-02-14 [PMID: 29445165]
• Bou Samra E, Buhagiar-Labarchede G, Machon C et al. A role for Tau protein in maintaining ribosomal DNA stability and cytidine deaminase-deficient cell survival. Nat Commun 2017-09-25 [PMID: 28947735]
• Gemble S, Buhagiar-Labarchede G, Onclercq-Delic R et al. A balanced pyrimidine pool is required for optimal Chk1 activation to prevent ultrafine anaphase bridge formation. J Cell Sci 2016-07-06 [PMID: 27383768]
• Kang X, Song C, Du X et al. PTEN stabilizes TOP2A and regulates the DNA decatenation. Sci Rep 2015-12-10 [PMID: 26657567]
• Luebben Spencer W, Kawabata Tsuyoshi, Johnson Charles S et al. A concomitant loss of dormant origins and FANCC exacerbates genome instability by impairing DNA replication fork progression. Nucleic Acids Res. 2014-03-03 [PMID: 24589582] (IF/IHC, Mouse)
• Munoz MC, Laulier C, Gunn A et al. RING finger nuclear factor RNF168 is important for defects in homologous recombination caused by loss of the breast cancer susceptibility factor BRCA1. J Biol Chem. 2012-11-23 [PMID: 23055523] (WB, Human)
• Rouzeau S, Cordelieres FP, Buhagiar-Labarchede G et al. Blooms Syndrome and PICH Helicases Cooperate with Topoisomerase IIalpha in Centromere Disjunction before Anaphase. PLoS One. 2012-04-26 [PMID: 22563370]
• Laulier C, Cheng A, Stark JM. The relative efficiency of homology-directed repair has distinct effects on proper anaphase chromosome separation Nucleic Acids Res 2011-08-01 [PMID: 21459848] (ICC/IF, Mouse, Human)

Review for ERCC6L Antibody (H00054821-D01P) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 05/11/2011

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse and human cell lines
• Species: Human

Bioinformatics

• Gene Symbol: ERCC6L
• Entrez:
  • 54821(Human)
• Uniprot:
  • AAH08808.1(Human)