TAO2 [p Ser181] Antibody - Azide Free

Western Blot: TAO2 [p Ser181] Antibody [NB300-233] - Western blot of rat hippocampal lysate showing specific labeling of the ~120 kDa TAO Kinase 2 (phospho-Ser181) band.

Western Blot: TAO2 [p Ser181] Antibody [NB300-233] - Western blot of rat cortex lysate showing specific immunolabeling of the ~120k TAO2 phosphorylated at Ser181 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubatede (1200 units for 30 min) before being exposed to the Ser181 TAO2 antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.

• Reactivity: Hu, Mu, Rt, Av, Ca, Ch, Av-Bi, Xp, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

TAO2 [p Ser181] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit TAO2 [p Ser181] Antibody - Azide Free (NB300-233) is a polyclonal antibody validated for use in WB. Anti-TAO2 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser181 conjugated to KLH. Accession # Q9UL54
• Modification: p Ser181
• Localization: Integral membrane protein. Found to be perinuclear and localized to vesicular compartment
• Specificity: Specific for endogenous levels of the ~120 kDa TAO2 phosphorylated at Ser181. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Predicted Species: Xenopus (100%), Canine (100%), Chicken (100%), Zebrafish (100%), Avian (100%), Finch (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TAOK2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Application Notes: Western The investigator should determine the optimal working dilution for a specific application.
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reactivity assumed based on 100% sequence homology: Finch.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for TAO2 [p Ser181] Antibody - Azide Free

• EC 2.7.11
• KFC-C
• KIAA0881PSK-1
• Kinase from chicken homolog C
• MAP3K17
• MAP3K17PSK1-BETA
• Prostate-derived STE20-like kinase 1
• PSK1
• PSK1EC 2.7.11.1
• PSKhKFC-C
• serine/threonine-protein kinase TAO2
• TAO kinase 2
• TAO1
• TAO2
• TAO2prostate derived STE20-like kinase PSK
• TAOK2
• Thousand and one amino acid protein 2
• thousand and one amino acid protein kinase

Background

In vitro, TAO (thousand and one amino acid) protein kinase 2 (TAO2) activates MAP/ERK kinases (MEKs) 3, 4, and 6 toward their substrates p38 MAP kinase JNK/SAPK (Chen et al., 1999; Chen and Cobb, 2001). This and more recent work has led to the proposal that the TAO protein kinases play an essential role in signaling from physiological agonists to the stress-responsive p38 MAPKs (Chen et al., 2003). Autophosphorylation of TAO may play a role in the mechanism of TAO activation. The MEK binding domain of TAO is autophosphorylated on both serine and threonine residues and Ser181 is located within this domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TAO2 Antibody (NB300-233)(5)

Publications using NB300-233

• Nsiah N Y, Morgan A B et al. Long-term HIF-1? stabilization reduces respiration, promotes mitophagy, and results in retinal cell death. Sci Rep 2024-11-23 [PMID: 37996657]
  
  Details:
  1:25
• Nsiah NY, Inman DM. Destabilizing COXIV in M�ller Glia Increases Retinal Glycolysis and Alters Scotopic Electroretinogram Cells 2022-11-24 [PMID: 36497016]
• Chen, Z, Raman, M, Chen, L, Lee, SF, Gilman, AG, Cobb, MH. TAO (thous--one amino acid) protein kinases mediate signaling from carbachol to p38 mitogen-activated protein kinase ternary complex factors, J. Biol Chem 278 22,278-22,283. 2003-01-01 [PMID: 12665513]
• Chen, Z, Cobb, MH. Regulation of stress-responsive mitogen-activated protein (MAP) kinase pathways by TAO2, J. Biol Chem 276 16,070-16,075. 2001-01-01 [PMID: 11279118]
• Chen, Z, Hutchison, M, Cobb, MH. Isolation of the protein kinase TAO2 identification of its mitogen-activated protein kinase/extracellular signal-regulated kinase kinase binding domain, J. Biol Chem 274 28,803-28,807. 1999-01-01 [PMID: 10497253]

Bioinformatics

• Gene Symbol: TAOK2
• Entrez:
  • 64666(Rat)
• Uniprot:
  • Q9UL54(Human)