ErbB4/Her4 Antibody (HFR-1)

Immunocytochemistry/Immunofluorescence: ErbB4/Her4 Antibody (HFR-1) [NBP3-07160] - Immunofluorescence Analysis of MCF-7 cells labeling HER-4 with ErbB4/Her4 Mouse Monoclonal Antibody (HFR-1) Goat anti- Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red).

Flow Cytometry: ErbB4/Her4 Antibody (HFR-1) [NBP3-07160] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using ErbB4/Her4 Mouse Monoclonal Antibody (HFR-1) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).

• Reactivity: Hu, Mu
• Applications: Flow, ICC/IF
• Clone: HFR-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ErbB4/Her4 Antibody (HFR-1) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08902)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant human ErbB4/Her4 protein fragment (around aa 1116-1269) (exact sequence is proprietary) (Uniprot: Q15303)
• Localization: Cytoplasmic, Cell Surface and Nuclear
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ERBB4
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.

Reactivity Notes

Predicted to work with Rat, Chicken.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for ErbB4/Her4 Antibody (HFR-1)

• avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4
• EC 2.7.10
• EC 2.7.10.1
• ErbB4
• HER4
• HER4MGC138404
• p180erbB4
• Proto-oncogene-like protein c-ErbB-4
• receptor tyrosine-protein kinase erbB-4
• Tyrosine kinase-type cell surface receptor HER4
• v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 4
• v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)

Background

erbB4/HER4 is a receptor tyrosine kinase that belongs to the human epidermal growth factor receptor (EGFR) family. erbB4/HER4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum (1). erbB4/HER4 is activated by neuregulins (NRG), betacellulin (BTC), and heparin-binding EGF-like growth factor (2). Neuregulins regulate the expression of ligand- and voltage-gated channels in neurons and skeletal muscle by the activation of ErbB 1-4. The erbB4/HER4 receptor is unique among these receptors because its C-terminal tail binds to a protein motif known as the PDZ domain (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ERBB4
• Uniprot:
  • Q15303()
  • Q15303()