ErbB2/Her2 Antibody (SP3)

Images

 
Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (SP3) [NBP1-49793] - IHC staining at 1:50 on HER+ human breast carcinoma, using a citrate antigen-retrieval buffer. Image from verified customer review.
Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (SP3) [NBP1-49793] - c-erbB-2/HER-2
Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (SP3) [NBP1-49793] Human Breast Carcinoma
Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (SP3) [NBP1-49793] - Human Breast.
Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (SP3) [NBP1-49793] - Human Bladder.
Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (SP3) [NBP1-49793] - Human Bladder.
Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (SP3) [NBP1-49793] - Human Placenta.
Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (SP3) [NBP1-49793] - Human Prostate Adenocarcinoma.
Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (SP3) [NBP1-49793] - Human Breast Ductal Carcinoma.

Product Details

Summary
Product Discontinued
View other related ErbB2/Her2 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-49793
    • Availability
      Product Discontinued

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ErbB2/Her2 Antibody (SP3) Summary

Immunogen
Recombinant protein encoding extracellular domain of human c-erbB2.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
ERBB2
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This product is useful for Immunohistochemistry Paraffin. Antigen retrieval can be performed using a Citrate process.
Theoretical MW
185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-49793 in the following applications:

Publications
Read Publication using
NBP1-49793 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27698900). Expected cross reactivity based on sequence identiy: Canine, Rat.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS (pH 7.5) and 1.0% BSA
Preservative
0.1% Sodium Azide
Purity
Tissue culture supernatant

Notes

For a positive control use Breast carcinoma.

Alternate Names for ErbB2/Her2 Antibody (SP3)

  • CD340 antigen
  • CD340
  • c-erb B2/neu protein
  • EC 2.7.10
  • EGFR2
  • ErbB2
  • HER2
  • HER-2
  • HER2EC 2.7.10.1
  • herstatin
  • Metastatic lymph node gene 19 protein
  • MLN 19
  • MLN19
  • Neu Oncogene
  • NEUHER-2/neu
  • neuroblastoma/glioblastoma derived oncogene homolog
  • NGL
  • NGLTKR1
  • p185erbB2
  • Proto-oncogene c-ErbB-2
  • Proto-oncogene Neu
  • receptor tyrosine-protein kinase erbB-2
  • TKR1
  • Tyrosine kinase-type cell surface receptor HER2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog)
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Background

The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Neu, a glycoprotein, undergoes transactivation upon heterodimerization with other EGF receptor family members. Neu heterodimerization with ErbB-3 recruits heregulin, which induces phosphoinositide (PI) 3-kinase activation. Activation of Neu potentiates tumor cell motility and protease secretion and invasion, and also modulates cell cycle checkpoint function, DNA repair and apoptotic responses. Amplification and/or overexpression of Neu occurs in 20-30% of breast carcinomas. Measurement of increased Neu expression can be a predictor of disease prognosis. Neu may also prove to be a promising target for therapeutic agents.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ErbB2/Her2 Antibody (NBP1-49793)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Mouse
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Review for ErbB2/Her2 Antibody (NBP1-49793) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IHC-P.

Reviews using NBP1-49793:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin ErbB2/Her2 NBP1-49793
Enlarge
reviewed by:
Nadia Hassounah
IHC-P Human 04/02/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman breast carcinoma
SpeciesHuman

Blocking

Blocking DetailsBlocked with 2.5% normal horse serum (Vector labs) and with Ventana Antibody Blocking Buffer and 5% goat serum

Primary Anitbody

Dilution Ratio1:50 overnight at 4 degrees, in Ventana Antibody Blocking Buffer

Secondary Antibody

Secondary DescriptionVector labs Immpress Reagent universal anti-mouse & anti-rabbit
Secondary Manufacturer Cat#MP-7500
Secondary ConcentrationPrediluted

Details

Detection NotesDAB was applied for 40 seconds.
Fixation DetailsCitrate buffer antigen retrieval using a pressure cooker
Wash DescriptionWash 3X 7 min with 1XPBS

Product General Protocols

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FAQs for ErbB2/Her2 Antibody (NBP1-49793). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
    • A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for ErbB2/Her2 Antibody (NBP1-49793)

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Diseases for ErbB2/Her2 Antibody (NBP1-49793)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Nadia Hassounah
04/02/2014
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol ERBB2
Entrez