ErbB2/Her2 Antibody (666521) [Unconjugated]

3T3‑L1 mouse embryonic fibroblast adipose-like cell line was stained with Rat Anti-Mouse ErbB2/Her2 Monoclonal Antibody (Catalog # MAB6744, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 666521
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

ErbB2/Her2 Antibody (666521) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse ErbB2/Her2
  Thr23-Thr653
  Accession # P70424
• Specificity: Detects mouse ErbB2/Her2 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human ErbB2, recombinant mouse (rm) ErbB3, rmErbB4, and rmEGF R is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: ERBB2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ErbB2/Her2 Antibody (666521) [Unconjugated]

• CD340 antigen
• CD340
• c-erb B2/neu protein
• EC 2.7.10
• EGFR2
• ErbB2
• HER2
• HER-2
• HER2EC 2.7.10.1
• herstatin
• Metastatic lymph node gene 19 protein
• MLN 19
• MLN19
• Neu Oncogene
• NEUHER-2/neu
• neuroblastoma/glioblastoma derived oncogene homolog
• NGL
• NGLTKR1
• p185erbB2
• Proto-oncogene c-ErbB-2
• Proto-oncogene Neu
• receptor tyrosine-protein kinase erbB-2
• TKR1
• Tyrosine kinase-type cell surface receptor HER2
• v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog)
• v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Background

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a 185 kDa type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors for EGF superfamily growth factors. ErbB2 is widely expressed in epithelial cells and plays roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The mouse ErbB2 extracellular domain (amino acids 23-653 of 1256) shares 85% and 94% aa identity with human and rat ErbB2 ECD, respectively. The protease ADAM10 releases a 110 kDa soluble fragment of ErbB2 from the cell surface. ErbB2 has no identified ligands, but heterodimerizes with ErbB1 (EGF R), ErbB3, or ErbB4 to form higher affinity signaling complexes. The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for ErbB2/Her2 Antibody (MAB6744). (Showing 1 - 1 of 1 FAQ).

1. I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
   • A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

Bioinformatics

• Gene Symbol: ERBB2
• Uniprot:
  • P70424()