ER beta/NR3A2 Antibody

Western Blot: ER beta/NR3A2 Antibody [NB120-3577] - Western blot analysis was performed on nuclear enriched extracts (30 ug lysate) of LNCap (Lane 1), PC-3 (Lane 2), MCF-7 (Lane 3), MDA-MB-231 (Lane 4), T-47D (Lane 5) and HeLa (Lane 6). The blots were probed with an anti-rabbit polyclonal ER beta/NR3A2 antibody [NB120-3577] at 1 ug/mL and detected by chemiluminescence using an HRP conjugated goat anti-rabbit IgG (H+L) secondary antibody at 0.4 ug/mL. A 60 kDa band corresponding to Estrogen Receptor beta was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using a 10% Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary antibody following blocking with 5% skim milk. Chemiluminescent detection was performed.

Immunocytochemistry/Immunofluorescence: ER beta/NR3A2 Antibody [NB120-3577] - Primate amygdala.

Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody [NB120-3577] - Figure 1 illustrates immunolocalization of ER beta in primate amygdala using NB120-3577.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ha, Pm, Sh
• Applications: WB, GS, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ER beta/NR3A2 Antibody Summary

• Immunogen: Synthetic peptide corresponding to residues A(55) E P Q K S P W C E A R S L E H(70) of rat ER beta.
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ESR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Gel Super Shift Assays 1 - 5 ul
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1 - 2 ug/ml
  • Immunohistochemistry-Frozen 1 - 2 ug/ml
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Western Blot 1 - 2 ug/ml
• Application Notes: WB: Detects an approx. 55 - 62 kDa protein representing ER beta from rat brain homogenate. ChIP usage was reported in the scientific literature (PMID: 19897598).
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 16153501). Bovine reactivity reported in scientific literature (PMID: 21397601). Hamster reactivity reported in scientific literature (PMID: 12943744).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ER beta/NR3A2 Antibody

• ER beta
• Erb
• ER-beta
• ESR2
• ESR-BETA
• ESTRB
• estrogen receptor 2 (ER beta)
• estrogen receptor beta 4
• estrogen receptor beta
• NR3A2
• NR3A2ESRB
• Nuclear receptor subfamily 3 group A member 2

Background

The human ER-

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ER beta/NR3A2 Antibody (NB120-3577)(12)

Publications using NB120-3577

• SimAo VA, Lupi JUnior LA, Adan Araujo Leite G et al. Nandrolone decanoate causes uterine injury by changing hormone levels and sex steroid receptors in a dose- and time-dependent manner Reproductive toxicology (Elmsford, N.Y.) 2021-06-01 [PMID: 33984419]
• Wang YX, Xia ZH, Jiang X et al. Genistein inhibits amyloid peptide 25-35-induced neuronal death by modulating estrogen receptors, choline acetyltransferase and glutamate receptors Arch. Biochem. Biophys. 2020-08-25 [PMID: 32857999] (ICC/IF, Rat)
• Ibrahim MMH, Bheemanapally K, Sylvester PW, Briski KP Sex differences in glucoprivic regulation of glycogen metabolism in hypothalamic primary astrocyte cultures: Role of estrogen receptor signaling Mol. Cell. Endocrinol. 2020-08-24 [PMID: 32853745] (WB, Rat)
• Briski KP, Ali MH, Napit PR Sex-Specific Acclimation of A2 Noradrenergic Neuron Dopamine-Beta-Hydroxylase and Estrogen Receptor Variant Protein and 5'-AMP-Activated Protein Kinase Reactivity to Recurring Hypoglycemia in Rat J. Chem. Neuroanat. 2020-06-26 [PMID: 32599255] (WB, Rat)
  
  Details:
  Sprague-Dawley rats
• Alshamrani AA, Bheemanapally K, Ibrahim MMH, Briski KP Impact of caudal hindbrain glycogen metabolism on A2 noradrenergic neuron AMPK activation and ventromedial hypothalamic nucleus norepinephrine activity and glucoregulatory neurotransmitter marker protein expression Neuropeptides 2020-05-16 [PMID: 32451071] (WB, Rat)
• Ibrahim MMH, Bheemanapally K, Sylvester PW, Briski KP Sex-specific estrogen regulation of hypothalamic astrocyte estrogen receptor expression and glycogen metabolism in rats Mol. Cell. Endocrinol. 2020-01-11 [PMID: 31931041] (WB, Rat)
• Uddin MM, Mahmood ASMH, Ibrahim MMH, Briski KP Sex-Dimorphic Estrogen Receptor Regulation of Ventromedial Hypothalamic Nucleus Glucoregulatory Neuron Adrenergic Receptor Expression in Hypoglycemic Male and Female Rats Brain Res. 2019-06-29 [PMID: 31265816] (WB, Rat)
• Abdullah, A, Talwar, P Et al. IRE1 alpha is critical for Kaempferol-induced neuroblastoma differentiation. FEBS J 2019-04-01 [PMID: 30719816] (IF/IHC, Mouse)
• Hasan Mahmood ASM, Mandal SK, Bheemanapally K et al. Norepinephrine control of ventromedial hypothalamic nucleus glucoregulatory neurotransmitter expression in the female rat: Role of monocarboxylate transporter function. Mol. Cell. Neurosci. 2019-01-17 [PMID: 30660767] (WB, Rat)
• Andrade G, Simao V, Souza B et al. Sex steroid receptors profiling is influenced by nandrolone decanoate in the ampulla of the fallopian tube: Post-treatment and post-recovery analyses Tissue and Cell 2018-02-01 [PMID: 29429521] (IHC-P, Rat)
• Simao VA, Chuffa LG, Camargo IC. Ovarian sex steroid receptors and sex hormones in androgenized rats. Reproduction 2016-08-25 [PMID: 27562815] (IF/IHC)
• Muhammad SI, Maznah I, Mahmud RB et al. Estrogen receptor modulatory effects of germinated brown rice bioactives in the uterus of rats through the regulation of estrogen-induced genes. Drug Des Devel Ther 2013-12-02 [PMID: 24324328] (IHC-P, Rat)

Bioinformatics

• Gene Symbol: ESR2
• Entrez:
  • 2100(Human)
  • 13983(Mouse)
  • 25149(Rat)
• Uniprot:
  • Q62986(Rat)