PP5 Antibody (OTI2E12)

Images

 
Western Blot: PP5 Antibody (2E12) [NBP1-47927] Analysis of extracts (35ug) from 9 different cell lines by using anti-PP5 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: PP5 Antibody (2E12) [NBP1-47927] - Immunofluorescent staining of HT29 cells using anti-PP5 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PP5 Antibody (2E12) [NBP1-47927] - Staining of paraffin-embedded Human pancreas tissue using anti-PP5 mouse monoclonal antibody.
Flow Cytometry: PP5 Antibody (2E12) [NBP1-47927] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PP5 antibody, and then analyzed by ...read more
Western Blot: PP5 Antibody (2E12) [NBP1-47927] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PP5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: PP5 Antibody (2E12) [NBP1-47927] - Staining of paraffin-embedded Human endometrium tissue using anti-PP5 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PP5 Antibody (2E12) [NBP1-47927] - Staining of paraffin-embedded Human Ovary tissue using anti-PP5 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI2E12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PP5 Antibody (OTI2E12) Summary

Immunogen
Full length human recombinant protein of human PPP5C (NP_006238) produced in HEK293T cell.
Specificity
This antibody is specific for Homo sapiens protein phosphatase 5, catalytic subunit (PPP5C).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PPP5C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000-2000
Theoretical MW
56.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PP5 Antibody (OTI2E12)

  • EC 3.1.3.16
  • PP5
  • PP5FLJ36922
  • PPP5
  • PPP5C
  • PPT
  • PP-T
  • protein phosphatase 5, catalytic subunit
  • Protein phosphatase T
  • Serine/Threonine phosphatase
  • serine/threonine-protein phosphatase 5

Background

May play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PP5 Antibody (NBP1-47927) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on PP5

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Bioinformatics

Gene Symbol PPP5C
Entrez