EPS15 Antibody [HRP]

Product Discontinued

EPS15 Antibody [HRP] Summary

• Immunogen: Synthetic peptide made to the C-terminal portion of human EPS15 (within residues 840-896). [Swiss-Prot# P42566]
• Localization: Cytoplasmic. Recruited to the plasma membrane upon EGFR activation and localizes to coated pits.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EPS15
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
• Application Notes: This EPS15 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western blot analysis, where a band is seen at ~135 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 135 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for EPS15 Antibody [HRP]

• AF1P
• AF-1P
• ALL1 fused gene from chromosome 1
• epidermal growth factor receptor pathway substrate 15
• epidermal growth factor receptor substrate 15
• Eps15
• MLLT5
• Protein AF-1p
• Protein Eps15

Background

EPS15 is a ubiquitously expressed protein that is involved in cell growth regulation and may be involved in the regulation of mitogenic signals and control of cell proliferation. Phosphorylation of EPS15 on Tyrosine 849 upon DNA damage is involved in the internalization of EGFR. A chromosomal aberration involving EPS15 is also found in acute leukemias, resulting in a rogue activator protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EPS15