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epithelial Sodium Channel gamma Antibody (3c7) [Alexa Fluor® 532]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P, S-ELISA
Clone
3c7
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 532

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-41371AF350
Alexa Fluor 405
NBP2-41371AF405
Alexa Fluor 488
NBP2-41371AF488
Alexa Fluor 594
NBP2-41371AF594
Alexa Fluor 647
NBP2-41371AF647
Alexa Fluor 700
NBP2-41371AF700
Alexa Fluor 750
NBP2-41371AF750
Biotin
NBP2-41371B
DyLight 350
NBP2-41371UV
DyLight 405
NBP2-41371V
DyLight 488
NBP2-41371G
DyLight 550
NBP2-41371R
DyLight 594
NBP2-41371DL594
DyLight 650
NBP2-41371C
DyLight 680
NBP2-41371FR
DyLight 755
NBP2-41371IR
FITC
NBP2-41371F
HRP
NBP2-41371H
Janelia Fluor 549
NBP2-41371JF549
Janelia Fluor 646
NBP2-41371JF646
Unconjugated
NBP2-41371
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-41371
BSA Free
NBP2-41371
BSA Free
NBP2-41371-0.025mg
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epithelial Sodium Channel gamma Antibody (3c7) [Alexa Fluor® 532] Summary

Immunogen
The inhibitory peptide from the human yENaC subunit. EAESWNSVSEGKQPRFSHRIPLC corresponding to amino acid residue 139-160 of human yENaC subunit.
Epitope
The epitope is on the inhibitory tract of human ENaC.
Specificity
Antibody is specific for the inhibitory tract of human gENaC subunit.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SCNN1G
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for epithelial Sodium Channel gamma Antibody (3c7) [Alexa Fluor® 532]

  • amiloride-sensitive epithelial sodium channel gamma subunit
  • amiloride-sensitive sodium channel gamma-subunit
  • BESC3
  • ENaC gamma subunit
  • ENaCG
  • ENaCgamma
  • Epithelial Na(+) channel subunit gamma
  • gamma-ENaC
  • gamma-NaCH
  • Nonvoltage-gated sodium channel 1 subunit gamma
  • PHA1
  • SCNEGamiloride-sensitive sodium channel subunit gamma
  • sodium channel, nonvoltage-gated 1, gamma

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry: epithelial Sodium Channel beta Antibody (14E10) [NBP2-59383] - Tissue: Kidney. Species: Rat. Fixation: Paraffin-embedded formalin-fixed. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:100. Secondary Antibody: Goat Anti-Mouse ATTO 488 (green). Localization: Intercalated cells. Aquaporin 2 Antibody staining in red.
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Western Blot: epithelial Sodium Channel alpha Antibody [NB100-74357] - Analysis of 25 ug of Daudi (lane 1) and mouse brain (lane 2) cell lysates onto an SDS polyacrylamide gel.
Immunocytochemistry/Immunofluorescence: epithelial Sodium Channel alpha Antibody [NB100-74357] - performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton(tm) X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha ENaC Rabbit Polyclonal Antibody at 2 ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal(tm) Secondary Antibody, Alexa Fluor(R) 488 conjugate a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade(R) Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor(R) 555 Rhodamine Phalloidin (1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Immunocytochemistry/Immunofluorescence: epithelial Sodium Channel alpha Antibody [NB100-74357] - performed using 70% confluent log phase HEK 293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton(tm) X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha ENaC Rabbit Polyclonal Antibody at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal(tm) Secondary Antibody, Alexa Fluor(R) 488 conjugate) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade(R) Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor(R) 555 Rhodamine Phalloidin (1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
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SLC12A3 Antibody
NBP1-44270
Western Blot: SLC12A3 Antibody [NBP1-44270] - analysis of Rat tissue lysates showing detection of NCC protein using Rabbit Anti-NCC Polyclonal Antibody. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:1000.
Immunocytochemistry/Immunofluorescence: SLC12A3 Antibody [NBP1-44270] - Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green).
Immunocytochemistry/Immunofluorescence: SLC12A3 Antibody [NBP1-44270] - analysis of NCC in rat kidney tissues, showing a 1:200 dilution of this antibody
(1 Publication)
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Applications: WB, EM, ICC/IF, IHC, IHC-P
CFTR Antibody
(24-1) [Unconjugated] ...
MAB25031
CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Mouse Anti-Human CFTR C‑Terminus Monoclonal Antibody (Catalog # MAB25031) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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(41 Publications)
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Mineralocorticoid
R/NR3C2 Antibody (H10 ...
NB300-562
Western Blot: Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) [NB300-562] - Analysis of Mineralocorticoid Receptor.
Immunocytochemistry/Immunofluorescence: Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) [NB300-562] - Mineralocorticoid Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mineralocorticoid Receptor at a dilution of 1:20-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) [NB300-562] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.
(1 Publication)
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NKCC2/SLC12A1
Antibody
NBP1-80993
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Immunocytochemistry/Immunofluorescence: NKCC2/SLC12A1 Antibody [NBP1-80993] - Staining of human cell line RPTEC TERT1 shows localization to nucleus & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-80993] - Analysis in human kidney and pancreas tissues using NBP1-80993 antibody. Corresponding SLC12A1 RNA-seq data are presented for the same tissues.
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SGK1 Antibody
(SC05-71)
NBP2-67561
Western Blot: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of SGK1 on human kidney lysates using anti-SGK1 antibody at 1/1,000 dilution.
Immunohistochemistry-Paraffin: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of paraffin-embedded mouse liver tissue using anti-SGK1 antibody. Counter stained with hematoxylin.
Flow Cytometry: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of 293 cells with SGK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Species: Hu, Mu, Rt
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Aquaporin-2
Antibody
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(2 Publications)
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Antibody
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Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-77461] - Vascularization of mineralized cell-laden collagen and interaction with prostate cancer cells. HUVECs formed endothelial networks that were supported by SMA-expressing hMSCs (scale bar: 50m) and were also positive for CD31 (scale bar: 400m). The remainder of hMSCs expressed RUNX2 as a marker for osteogenic differentiation (scale bar: 50m). Rapid fabrication of vascularized and innervated cell-laden bone models with biomimetic intrafibrillar collagen mineralization. Nat Commun (2019)
Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody [NBP1-77461] - RUNX2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-77461] - Characterization of TLC cultured in chondrogenic pellets. Gene expression of TLC cultured four weeks in chondrogenic pellets or in cell monolayer in control medium: levels of expression of osteoblast markers (1), chondrocyte markers (2) and tenocyte markers (3) (N = 3) assessed by qPCR.* =0.05. Rotator Cuff Tenocytes Differentiate into Hypertrophic Chondrocyte-Like Cells to Produce Calcium Deposits in an Alkaline Phosphatase-Dependent Manner. J Clin Med (2019)
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Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Renin was detected in immersion fixed paraffin-embedded sections of human kidney array using Sheep Anti-Human Renin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4090) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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NB100-62346
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Immunohistochemistry: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Protein expressions of intrarenal RAS components in two groups of mice. (A) Immunohistochemical analysis of intrarenal RAS expression position in the two groups of mice. AGT immunoreactivity in the proximal tubular cells, Ang II immunoreactivity in both glomerular and tubular cells, renin immunoreactivity in juxtaglomerular apparatus cells, ACE immunoreactivity in brush border membranes of proximal tubules as well as AT1 and AT2 immunoreactivity in the proximal tubules were increased in the HF group when compared to the control group. Image collected and cropped by CiteAb from the following publication (lipidworld.biomedcentral.com/articles/10.1186/1476-511X-12-49), licensed under a CC-BY licence.
Western Blot: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Evaluation of angiotensis II expression in untreated UUO rat kidneys and UUO rat kidneys treated with Ang-(1-7).
(13 Publications)
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P
ERMAP Antibody
AF4928
K562 human chronic myelogenous leukemia cell line was stained with Goat Anti-Human ERMAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4928, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Species: Hu
Applications: WB, Flow, CyTOF-ready
epithelial Sodium
Channel gamma Antibod ...
NBP2-41371AF532
epithelial Sodium Channel gamma Antibody (3c7) [Alexa Fluor® 532]
Species: Hu
Applications: WB, ELISA, IHC, IHC-Fr, IHC-P, S-ELISA

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-41371AF350
Alexa Fluor 405 NBP2-41371AF405
Alexa Fluor 488 NBP2-41371AF488
Alexa Fluor 532 NBP2-41371AF532
Alexa Fluor 594 NBP2-41371AF594
Alexa Fluor 647 NBP2-41371AF647
Alexa Fluor 700 NBP2-41371AF700
Alexa Fluor 750 NBP2-41371AF750
Biotin NBP2-41371B
DyLight 350 NBP2-41371UV
DyLight 405 NBP2-41371V
DyLight 488 NBP2-41371G
DyLight 550 NBP2-41371R
DyLight 594 NBP2-41371DL594
DyLight 650 NBP2-41371C
DyLight 680 NBP2-41371FR
DyLight 755 NBP2-41371IR
FITC NBP2-41371F
HRP NBP2-41371H
Janelia Fluor 549 NBP2-41371JF549
Janelia Fluor 646 NBP2-41371JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol SCNN1G