epithelial Sodium Channel gamma Antibody (3c7) - BSA Free

Western Blot: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371] - Analysis on a lysate of HEK293 cells transfected with and without yENaC. Lane 1 loaded with 5ug Lane 2 loaded with 10ug Lane 3 loaded with 20ug

ELISA: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371] - The calibration curve of a sandwich assay for Anti-gENaC using NBP2-41371 as the capture antibody and NBP2-41373B as the biotinylated detection antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, ELISA
• Clone: 3c7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

epithelial Sodium Channel gamma Antibody (3c7) - BSA Free Summary

• Description: Novus Biologicals Mouse epithelial Sodium Channel gamma Antibody (3c7) - BSA Free (NBP2-41371) is a monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The inhibitory peptide from the human gamma-ENaC subunit. EAESWNSVSEGKQPRFSHRIPLC corresponding to amino acid residue 139-160 of human gamma-ENaC subunit.
• Epitope: The epitope is on the inhibitory tract of human ENaC.
• Specificity: Antibody is specific for the inhibitory tract of human gamma-ENaC subunit.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SCNN1G
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA Capture 1-4 ug/ml
  • Sandwich ELISA
  • Western Blot 1:100-1:2000
• Application Notes: ELISA: NBP2-41371 was used in ELISA. A sandwich ELISA can be made using NBP2-41371 (1-4 ug/ml) as the capture antibody and biotinylated NBP2-41373 (0.05 - 0.2 ug/ml) as the detection antibody in order to detect the peptide from the inhibitory tract (AA 138-131). WB: NBP2-41371 was used in Western blot. (1,2) IHC: NBP2-41371 was used in immunohistochemistry.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for epithelial Sodium Channel gamma Antibody (3c7) - BSA Free

• amiloride-sensitive epithelial sodium channel gamma subunit
• amiloride-sensitive sodium channel gamma-subunit
• BESC3
• ENaC gamma subunit
• ENaCG
• ENaCgamma
• Epithelial Na(+) channel subunit gamma
• Gamma-ENaC
• Gamma-NaCH
• Nonvoltage-gated sodium channel 1 subunit gamma
• PHA1
• SCNEGamiloride-sensitive sodium channel subunit gamma
• sodium channel, nonvoltage-gated 1, gamma

Background

Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the gamma subunit, and mutations in this gene have been associated with Liddle syndrome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCNN1G