Recombinant Human Epiregulin Protein

Product Discontinued

Recombinant Human Epiregulin Protein Summary

• Description: Recombinant bioactive protein containing 50 amino acids for Human Epiregulin
  
  

  Source:E. coli
  Amino Acid Sequence:(O14944)

• Preparation Method: Determined to be >97% pure by SDS-PAGE
• Details of Functionality: The ED50 as dertermined by the dose-dependent stimulation of the proliferation of murine balb/3T3 cells is <2.0 ng/ml, corresponding to a specific activity of >5 x 105 units/mg
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: EREG
• Purity: >97%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 20 mM PBS (pH 7.4), 130 mM NaCl.
• Concentration: Lyoph
• Purity: >97%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in dH2O to a concentration of 0.1 - 1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for long-term storage.

Alternate Names for Recombinant Human Epiregulin Protein

• Epiregulin
• ER
• EREG
• proepiregulin

Background

Epiregulin is part of the EGF family. Epiregulin functions as a ligand of EGFR, as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. Epiregulin is expressed mostly in the placenta and peripheral blood leukocytes and in specific carcinomas of the bladder, lung, kidney and colon. Epiregulin stimulates the proliferation of keratinocytes, hepatocytes, fibroblasts and Vascular smooth muscle cells. Epiregulin inhibits the growth of several tumor-derived epithelial cell lines. Human Epiregulin is initially synthesized as a glycosylated 19.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a 6.0 kDa mature secreted sequence. Recombinant human EREG produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 50 amino acids and having a molecular mass of 6 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: EREG