Epiregulin Antibody (189611) [Alexa Fluor® 405]

• Reactivity: Mu
• Applications: B/N, ELISA(Cap), ELISA(Det)
• Clone: 189611
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 405

Epiregulin Antibody (189611) [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant mouse Epiregulin
  Val56-Leu101
  Accession # Q61521
• Specificity: Detects mouse Epiregulin in ELISAs. In direct ELISAs, 100% cross‑reactivity with recombinant human Epiregulin is observed, but no cross‑reactivity with recombinant mouse (rm) Epigen, recombinant human (rh) TGF-alpha , rhAmphiregulin, r
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • Neutralization

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Epiregulin Antibody (189611) [Alexa Fluor® 405]

• Epiregulin
• ER
• EREG
• proepiregulin

Background

Epiregulin is a member of the EGF family of growth factors which includes, among others, epidermal growth factor (EGF), transforming growth factor (TGF)-alpha , amphiregulin (ARG), HB (heparin-binding)-EGF, betacellulin, and the various heregulins. They are all synthesized as transmembrane precursors and converted to soluble forms by proteolytic cleavage. Epiregulin was originally purified from the mouse fibroblast-derived tumor cell line NIH3T3/T7 (1). The mouse Epiregulin cDNA encodes for a 162 amino acid (aa) transmembrane precursor, with the mature soluble form comprising aa 56‑101 (2). The mode of action of Epiregulin is similar to other EGF family members in that it binds to and activates the tyrosine-kinase, ErbB-family receptors (ErbB1 through B4) (3). Although it stimulates phosphorylation of all four receptors, it appears to interact primarily with ErbB1 and ErbB4. Epiregulin has the broadest specificity of the EGF-like ligands but seems to preferentially activate heterodimeric receptor complexes (4). Epiregulin exhibits a variety of biological effects. It was originally shown to both inhibit growth of several epithelial tumor cells and stimulate growth of fibroblasts and other types of cells (1). Epiregulin expression is upregulated in a number of carcinoma cell lines. It has also been shown to be an autocrine growth factor in human epidermal keratinocytes (5). Epiregulin has also been shown to play a role in the early steps of pregnancy, regulating attachment of the blastocyst to the uterine epithelium during the implantation process (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.