Reactivity | HuSpecies Glossary |
Applications | ELISA |
Suitable Sample Type | Cell lysates, tissue homogenates and other biological fluids |
Standard Curve Range | 0.156-10 ng/ml |
Sensitivity | 0.078 ng/ml |
Description | The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human Ephrin-B2 in the sample, this Human Ephrin-B2 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human Ephrin-B2 concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve. |
Standard Curve Range | 0.156-10 ng/ml |
Sensitivity | 0.078 ng/ml |
Assay Type | Sandwich ELISA |
Inter-Assay | %CV < 15 |
Intra-Assay | %CV < 10 |
Sample Volume | 100 ul |
Kit Type | ELISA Kit (Colorimetric) |
Gene | EFNB2 |
Storage | Storage of components varies. See protocol for specific instructions. |
Components |
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Gene Symbol | EFNB2 |