![Sandwich ELISA: EphB6 Antibody Pair [HRP] [NBP2-79490] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize2/EphB6-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79490-img0001.jpg "Sandwich ELISA: EphB6 Antibody Pair [HRP] [NBP2-79490] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, S-ELISA |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | HRP |
| Standard Curve Range | 9.38-600 pg/ml (example only; lot dependent) |
| Sensitivity | 9.4 pg/ml (example only; lot dependent) |
| Description | Solid Phase sandwich ELISA for the quantitative determination of Human EphB6. |
| Additional Information | Recombinant Monoclonal Antibody |
| Specificity | This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human EphB6. |
| Standard Curve Range | 9.38-600 pg/ml (example only; lot dependent) |
| Sensitivity | 9.4 pg/ml (example only; lot dependent) |
| Assay Type | Sandwich ELISA |
| Sample Volume | 100 ul |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | EPHB6 |
| Dilutions |
|
| Application Notes | 1. Capture Antibody (NBP2-89326): Dilute to a working concentration of 2 ug/ml in CBS before coating. 2. Capture Antibody (NBP2-89327): Dilute to working concentration of 0.5 ug/ml in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.6 ng/ml is recommended. |
| Storage | Storage of components varies. See protocol for specific instructions. |
| Components |
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![Immunohistochemistry-Paraffin: Ceruloplasmin Antibody (6C3K9) [NBP3-15868] - Immunohistochemistry of paraffin-embedded rat liver using Ceruloplasmin Rabbit mAb (NBP3-15868) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/images2/Ceruloplasmin-Antibody-(6C3K9)-Immunohistochemistry-Paraffin-NBP3-15868-img0001.jpg)
![Immunohistochemistry-Paraffin: Ceruloplasmin Antibody (6C3K9) [NBP3-15868] - Immunohistochemistry of paraffin-embedded human liver using Ceruloplasmin Rabbit mAb (NBP3-15868) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/images2/Ceruloplasmin-Antibody-(6C3K9)-Immunohistochemistry-Paraffin-NBP3-15868-img0002.jpg)
![Immunohistochemistry: Ceruloplasmin Antibody (6C3K9) [NBP3-15868] - Immunofluorescence analysis of rat liver using Ceruloplasmin Rabbit mAb (NBP3-15868) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/Ceruloplasmin-Antibody-(6C3K9)-Immunohistochemistry-NBP3-15868-img0003.jpg)
![Flow Cytometry: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MEK4 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/images2/MKK4-MEK4-Antibody-(OTI8A8)-Flow-Cytometry-NBP1-47839-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of HepG2 cells using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/images2/MKK4-MEK4-Antibody-(OTI8A8)-Immunocytochemistry-Immunofluorescence-NBP1-47839-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEK4.](http://images.novusbio.com/images2/MKK4-MEK4-Antibody-(OTI8A8)-Immunocytochemistry-Immunofluorescence-NBP1-47839-img0013.jpg)
![Flow Cytometry: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Analysis of Hela cells with Phospho-JNK1/2/3(T183+T183+T221) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the second](http://images.novusbio.com/images2/JNK1-[p-Thr183--p-Thr221]-Antibody-(ST500)-Flow-Cytometry-NBP2-67514-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Staining Phospho-JNK1/2/3(T183+T183+T221) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/JNK1-[p-Thr183--p-Thr221]-Antibody-(ST500)-Immunocytochemistry-Immunofluorescence-NBP2-67514-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: JNK1 [p Thr183, p Thr221] Antibody (ST500) [NBP2-67514] - Staining Phospho-JNK1/2/3(T183+T183+T221) in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/JNK1-[p-Thr183--p-Thr221]-Antibody-(ST500)-Immunocytochemistry-Immunofluorescence-NBP2-67514-img0003.jpg)
![Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Feline corneal stromal cells. IHC-Fr and IF (Alexa Fluor488) were performed using NBP1-91258 at 1:400. Image captured by epifluorescent microscopy. Image from verified customer review.](http://images.novusbio.com/images2/Fibronectin-Antibody-Immunohistochemistry-Frozen-NBP1-91258-img0013.jpg)
![Western Blot: Fibronectin Antibody [NBP1-91258] - Analysis of Fibronectin in HepG2 cell lysate.](http://images.novusbio.com/images/Fibronectin-Antibody-Western-Blot-NBP1-91258-img0003.jpg)
![Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Trabecular meshwork (TM) region of pig eyes. NBP1-91258 Fibronectin antibody was labelled with Alexa Fluor 488 conjugated secondary antibody (Green). DAPI shown as blue. Image from verified customer review.](http://images.novusbio.com/images2/Fibronectin-Antibody-Immunohistochemistry-Frozen-NBP1-91258-img0016.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.](http://images.novusbio.com/images2/ER-alpha-NR3A1-Antibody-(33)-Western-Blot-NB300-560-img0004.jpg)
![Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/images2/ER-alpha-NR3A1-Antibody-(33)-Immunohistochemistry-Frozen-NB300-560-img0009.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/images2/ER-alpha-NR3A1-Antibody-(33)-Immunohistochemistry-Paraffin-NB300-560-img0010.jpg)
![Flow Cytometry: MKK7/MEK7 Antibody (SD20-87) [NBP2-67764] - Analysis of Hela cells with MEK7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/images2/MKK7-MEK7-Antibody-(SD20-87)-Flow-Cytometry-NBP2-67764-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MKK7/MEK7 Antibody (SD20-87) [NBP2-67764] - Staining MEK7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/MKK7-MEK7-Antibody-(SD20-87)-Immunocytochemistry-Immunofluorescence-NBP2-67764-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MKK7/MEK7 Antibody (SD20-87) [NBP2-67764] - Staining MEK7 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/MKK7-MEK7-Antibody-(SD20-87)-Immunocytochemistry-Immunofluorescence-NBP2-67764-img0003.jpg)
![Western Blot: GTP binding protein era homolog Antibody [NBP1-89703] - Analysis using Anti-ERAL1 antibody NBP1-89703 (A) shows similar pattern to independent antibody NBP1-89704 (B).](http://images.novusbio.com/images2/GTP binding protein era homolog Antibody-Western Blot-NBP1-89703-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: GTP binding protein era homolog Antibody [NBP1-89703] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & mitochondria.](http://images.novusbio.com/images2/GTP-binding-protein-era-homolog-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89703-img0006.jpg)
![Immunohistochemistry-Paraffin: GTP binding protein era homolog Antibody [NBP1-89703] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/GTP-binding-protein-era-homolog-Antibody-Immunohistochemistry-Paraffin-NBP1-89703-img0002.jpg)
![Knockdown Validated: Calreticulin Antibody [NB600-101] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1500 of Rabbit Anti-Human Calreticulin Polyclonal Antibody (Catalog # NB600-101) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Calreticulin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/Calreticulin-Antibody-Knockdown-Validated-NB600-101-img0011.jpg)
![Western Blot: Calreticulin Antibody [NB600-101] - Human kidney lysate.](http://images.novusbio.com/images2/Calreticulin-Antibody-Western-Blot-NB600-101-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [NB600-101] - The Calreticulin antibody NB600-101 was tested in HeLa cells at a 1:250 dilution against DyLight 488 (Green). Alpha-tubulin and nuclei were counterstained against DyLight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/images2/Calreticulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-101-img0007.jpg)
Diseases for EphB6 Antibody Pair (NBP2-79490)Discover more about diseases related to EphB6 Antibody Pair (NBP2-79490).
| Pathways for EphB6 Antibody Pair (NBP2-79490)View related products by pathway.
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| Gene Symbol | EPHB6 |
![Immunohistochemistry-Paraffin: UROD Antibody [NBP2-26190] - (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/images2/UROD-Antibody-Immunohistochemistry-Paraffin-NBP2-26190-img0002.jpg)
![Western Blot: UROD Antibody [NBP2-26190] - Staining of K562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/images2/UROD-Antibody-Western-Blot-NBP2-26190-img0001.jpg)
![Ephrin-B2 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Ephrin-B2 [Unconjugated]")
![Immunocytochemistry/Immunofluorescence: Albumin Antibody [NB600-41532] - NLRP12 downregulates multiple JNK activating pathways in hepatocytes. For checking the purity of primary hepatocytes, hepatocytes isolated from healthy WT and Nlrp12-/- mouse livers were immunostained with antibody for hepatocyte-specific marker Albumin and counterstained with DAPI. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/40396), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Albumin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-41532-img0003.jpg)
![Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. WB image submitted by a verified customer review.](http://images.novusbio.com/images2/Albumin-Antibody-Western-Blot-NB600-41532-img0002.jpg)
