EphB6 Antibody (5D8) - Azide and BSA Free

Western Blot: EphB6 Antibody (5D8) [H00002051-M03] - Analysis of EPHB6 expression in NIH/3T3 (Cat # L018V1).

Immunohistochemistry-Paraffin: EphB6 Antibody (5D8) [H00002051-M03] - Analysis of monoclonal antibody to EPHB6 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml

Western Blot: EphB6 Antibody (5D8) [H00002051-M03] - Analysis of EPHB6 expression in PC-12 (Cat # L012V1).

ELISA: EphB6 Antibody (5D8) [H00002051-M03] - Detection limit for recombinant GST tagged EPHB6 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clone: 5D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

EphB6 Antibody (5D8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: EPHB6 (NP_004436, 23 a.a. ~ 122 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DTTGETSEIGWLTYPPGGWDEVSVLDDQRRLTRTFEACHVAGAPPGTGQDNWLQTHFVERRGAQRAHIRLHFSVRACSSLGVSGGTCRETFTLYYRQAEE
• Specificity: EPHB6 - EPH receptor B6
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EPHB6
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EphB6 Antibody (5D8) - Azide and BSA Free

• EC 2.7.10.1
• EPH receptor B6
• EphB6
• ephrin type-B receptor 6
• Hep
• HEPTyrosine-protein kinase-defective receptor EPH-6
• Mep
• MGC129910
• MGC129911

Background

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ephrin receptor encoded by this gene lacks the kinase activity of most receptor tyrosine kinases and binds to ephrin-B ligands.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EphB6 Antibody (H00002051-M03)(1)

Publication using H00002051-M03

• Bailey CM, Kulesa PM. Dynamic Interactions between Cancer Cells and the Embryonic Microenvironment Regulate Cell Invasion and Reveal EphB6 as a Metastasis Suppressor. Mol Cancer Res. 2014-05-16 [PMID: 24836890]

Review for EphB6 Antibody (H00002051-M03) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Diego Arango WB Human 01/13/2012

Summary

• Application: Western Blot
• Sample Tested: SW480 whole cell lysate, Sample Amount: 100ug
• Species: Human

Bioinformatics

• Gene Symbol: EPHB6
• Entrez:
  • 2051(Human)
• OMIM:
  • 602757(Human)
• Uniprot:
  • NP_004436()