ENPP-7/Alk-SMase Antibody

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ENPP-7/Alk-SMase Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ENPP‑7/Alk‑SMase
  Ala22-Ser439
  Accession # Q6UWV6
• Specificity: Detects human ENPP‑7/Alk‑SMase in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse ENPP-7 is observed, and less than 5% cross-reactivity with recombinant human (rh) ENPP-2 and rhENPP-5 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ENPP7
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ENPP-7/Alk-SMase Antibody

• Alkaline sphingomyelin phosphodiesterase
• alkaline sphingomyelinase
• Alk-SMase
• EC 3.1.4.12
• ectonucleotide pyrophosphatase/phosphodiesterase 7
• ectonucleotide pyrophosphatase/phosphodiesterase family member 7
• E-NPP 7
• ENPP7
• ENPP-7
• Intestinal alkaline sphingomyelinase
• MGC50179
• NPP7
• NPP-7

Background

ENPP-7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (alk-SMase) is expressed in the intestines and in human bile (1). It shares 30%‑36% homology with the members of the nucleotide pyrophosphatase/phosphodiesterese (NPP) family while sharing no similarities with neutral or acid SMase (2). Its main function is the digestion of dietary sphingomyelin by hydrolyzing sphingomyelin into ceramide and phosphorylcholine. ENPP-7 is reported to hydrolyse and inactivate platelet-activating factor (PAF) by a phospholipase C-type activity (3). Studies also show a decrease in ENPP-7 activity in human colorectal adenocarcinomas and human colorectal carcinomas, which indicate a potential role of ENPP-7 in human colon cancer (4, 5).

1. Duan, R-D. et al. (2006) Biochim. Biophys. 1761:281.
2. Duan, R-D. et al. (2003) J. Biol. Chem. 278:38528.
3. Wu, J. et al. (2006) Biochem J. 394:299.
4. Hertervig, E. et al. (1996) Cancer. 79:448.
5. Hertervig, E. et al. (1999) Br. J. Cancer. 81:232.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENPP7
• Uniprot:
  • Q6UWV6()