Enolase 2/Neuron-specific Enolase Antibody

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Immunohistochemistry-Paraffin: NSE Antibody [NB120-834] - Staining of Human pancreas

Product Details

Summary
Reactivity Hu, BvSpecies Glossary
Applications IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Enolase 2/Neuron-specific Enolase Antibody Summary

Immunogen
Neuron specific enolase (gamma-gamma isoenzyme) isolated from bovine brain.
Localization
Cytoplasmic
Marker
Neuronal Marker
Specificity
This antibody reacts with human isoenzymes of neuron specific enolase containing gamma subunits. It gives a specific staining in neurons of the brain, islets of Langerhans in pancreas, and in peripheral nerves of the skin. No staining with other tissue types has been seen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ENO2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
IHC: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
Positive Control
Enolase 2/Neuron-specific Enolase Lysate (NBL1-10268)

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Whole antisera
Preservative
0.1% Sodium Azide
Purity
Unpurified

Notes

This product is available in a small concetrated size as well as a larger pre-dilute size. The larger can be used neat without further dilution.

Alternate Names for Enolase 2/Neuron-specific Enolase Antibody

  • 2-phospho-D-glycerate hydrolyase
  • 2-phospho-D-glycerate hydro-lyase
  • EC 4.2.1.11
  • ENO2
  • enolase 2 (gamma, neuronal)
  • Enolase 2
  • gamma-Enolase
  • Neural enolase
  • neuron specific gamma enolase
  • neurone-specific enolase
  • Neuronspecific Enolase
  • Neuron-specific enolase
  • NSE

Background

Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ENO2
Entrez