Enolase 2/Neuron-specific Enolase Antibody

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody [NB120-834] - Formalin fixed paraffin embedded human pancreas stained with Enolase 2/Neuron-specific Enolase antibody (NB120-834).

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody [NB120-834] - Staining of Human pancreas

Product Discontinued

Enolase 2/Neuron-specific Enolase Antibody Summary

• Immunogen: Neuron specific enolase (gamma-gamma isoenzyme) isolated from bovine brain.
• Localization: Cytoplasmic
• Marker: Neuronal Marker
• Specificity: This antibody reacts with human isoenzymes of neuron specific enolase containing gamma subunits. It gives a specific staining in neurons of the brain, islets of Langerhans in pancreas, and in peripheral nerves of the skin. No staining with other tissue types has been seen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ENO2
• Purity: Whole Antiserum

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.
• Control:

  Enolase 2/Neuron-specific Enolase Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Whole Antiserum
• Preservative: 0.05% Sodium Azide
• Purity: Whole Antiserum

Notes

This product is available in a small concetrated size as well as a larger pre-dilute size. The larger can be used neat without further dilution.

Alternate Names for Enolase 2/Neuron-specific Enolase Antibody

• 2-phospho-D-glycerate hydrolyase
• 2-phospho-D-glycerate hydro-lyase
• EC 4.2.1.11
• ENO2
• enolase 2 (gamma, neuronal)
• Enolase 2
• gamma-Enolase
• Neural enolase
• neuron specific gamma enolase
• neurone-specific enolase
• Neuronspecific Enolase
• Neuron-specific enolase
• NSE

Background

Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENO2
• Entrez:
  • 2026(Human)