Enolase 1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Enolase 1 Antibody [NB100-65252] - Staining of Jurkat cells with rabbit anti human Enolase 1.
Simple Western: Enolase 1 Antibody [NB100-65252] - Simple Western lane view shows a specific band for Enolase 1 in 0.2 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, BvSpecies Glossary
Applications WB, Simple Western, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
5 mg/ml

Order Details

Enolase 1 Antibody Summary

Immunogen
Native human Enolase 1
Localization
Cytoplasm. Cell membrane. Isoform MBP-1: Nucleus. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
Specificity
This recognises human Non-neuronal enolase, and demostrates less than 0.1% cross-reactivity with non-specific enolase. Non-neuronal enolase is a glycolytic enzyme composed of homodimeric alpha enolase subunits and is expressed in most human tissues. The gene coding for the 48kDa form of non-neuronal enolase also codes for a shorter 37kDa isoform, myc-binding protein 1 (MBP-1) lacking the N-terminal 93 amino acids of non-neuronal enolase.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ENO1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Simple Western 1:5000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:25-1:100
  • Immunohistochemistry-Paraffin 1:25-1:100
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Publications
Read Publication using
NB100-65252 in the following applications:

  • WB
    1 publication

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 21051714).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
5 mg/ml
Purity
Protein G purified

Alternate Names for Enolase 1 Antibody

  • 2-phospho-D-glycerate hydro-lyase
  • Alpha enolase
  • C-myc promoter-binding protein
  • c-myc promoter-binding protein-1
  • EC 4.2.1
  • EC 4.2.1.11
  • ENO1L1
  • Enolase 1
  • enolase 1, (alpha)
  • MBP-1
  • MBPB1
  • MPB-1
  • MPB1c-myc promoter-binding protein 1
  • MYC promoter-binding protein 1
  • NNE
  • Non-neural enolase
  • Phosphopyruvate hydratase
  • Plasminogen-binding protein
  • PPHalpha enolase like 1
  • tau-crystallin

Background

ENO1 encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Enolase 1 Antibody (NB100-65252)(1)

We have publications tested in 1 confirmed species: Bovine.

We have publications tested in 1 application: WB.


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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ENO1
Entrez
Uniprot