Endothelin 2 Overexpression Lysate (Native)


Western Blot: Endothelin 2 Overexpression Lysate (Adult Normal) [NBL1-10114] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Endothelin 2.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

Endothelin 2 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC206602 and based on accession number NM_001956. The protein contains a C-terminal DDK tag.
Homo sapiens endothelin 2 (EDN2), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
17.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for Endothelin 2

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Endothelin 2 Overexpression Lysate (Native)

  • EDN2
  • endothelin 2
  • Endothelin2
  • endothelin-2
  • ET2
  • ET-2
  • PPET2
  • preproendothelin 2
  • preproendothelin-2


This gene encodes a member of the endothelin protein family of secretory vasoconstrictive peptides. The preproprotein is processed to a short mature form which functions as a ligand for the endothelin receptors that initiate intracellular signaling events. This gene product is involved in a wide range of biological processes, such as hypertension and ovulation. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, ELISA, IP, S-ELISA
Species: Hu, Mu, Rt, Po, Ca, Rb, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, RIA
Species: Hu, Ca
Applications: WB, IHC-P, ICC
Species: Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, IHC
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: IHC, IHC-P
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, GP, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Endothelin 2 Lysate (NBL1-10114) (0)

There are no publications for Endothelin 2 Lysate (NBL1-10114).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Endothelin 2 Lysate (NBL1-10114) (0)

There are no reviews for Endothelin 2 Lysate (NBL1-10114). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Endothelin 2 Lysate (NBL1-10114) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Endothelin 2 Products

Related Products by Gene

Bioinformatics Tool for Endothelin 2 Lysate (NBL1-10114)

Discover related pathways, diseases and genes to Endothelin 2 Lysate (NBL1-10114). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Endothelin 2 Lysate (NBL1-10114)

Discover more about diseases related to Endothelin 2 Lysate (NBL1-10114).

Pathways for Endothelin 2 Lysate (NBL1-10114)

View related products by pathway.

PTMs for Endothelin 2 Lysate (NBL1-10114)

Learn more about PTMs related to Endothelin 2 Lysate (NBL1-10114).

Research Areas for Endothelin 2 Lysate (NBL1-10114)

Find related products by research area.

Blogs on Endothelin 2

There are no specific blogs for Endothelin 2, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol EDN2

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBL1-10114 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

GFAP Antibody
GFAP Antibody
GFAP Antibody