Endothelin-1 Quantikine ELISA Kit

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Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications ELISA
Conjugate
HRP

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Endothelin-1 Quantikine ELISA Kit Summary

Background
The Quantikine Endothelin-1 immunoassay is a 4.5 hour solid phase ELISA designed to measure ET-1 in cell culture supernates, serum, plasma, and urine. It contains synthetic ET-1 and antibodies raised against synthetic ET-1. This immunoassay has been shown to accurately quantitate synthetic and naturally occurring ET-1.
Specificity
Synthetic and natural Endothelin-1
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DET100.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Endothelin-1 Quantikine ELISA Kit

  • ARCND3
  • EDN1
  • endothelin 1
  • Endothelin-1
  • ET1
  • HDLCQ7
  • PPET1
  • preproendothelin-1
  • QME

Background

Endothelin-1 (ET-1), a peptide of 21 amino acid (aa) residues, is a pleiotropic molecule best known for its action as a potent vasoconstrictor (1). Originally isolated from porcine aortic endothelial cells, ET-1 is one of a family of three proteins encoded by distinct genes that also includes Endothelin-2 (ET-2) and Endothelin-3 (ET-3) (2, 3). ET-2 and ET-3 differ from ET-1 by 2 and 6 amino acids, respectively (1, 2). All members of the Endothelin family contain two essential disulfide bridges and six conserved aa residues at the C-terminus. Human ET-1 is initially synthesized as a pre-pro-polypeptide of 212 amino acids (2, 4). It is proteolytically cleaved by a signal peptidase to produce pro-ET-1 and further processed by a Furin-like protease to yield a 38 aa peptide termed Big ET-1 (5, 6). Big ET-1 is then cleaved by the membrane-bound metalloprotease Endothelin-converting enzyme (ECE-1), producing the potent 21 aa mature form ET-1 (aa 1-21) (7, 8). Alternatively, ET-1 may exist in an active 31 aa form (ET-1 (aa 1-31)) following cleavage of Big ET-1 by chymase (9-12). The vascular endothelium is an abundant source of ET-1 (3, 13). It may also be expressed by leukocytes, smooth muscle cells, mesangial cells, cardiac myocytes, and astrocytes (14, 15). ET-1 can be induced in endothelial cells by many factors including mechanical stimulation, various hormones, and pro-inflammatory cytokines (16). Production is inhibited by nitric oxide (NO), Prostacyclin, and atrial natriuretic peptide (ANP) (17-19). 
Two receptors for the Endothelin family have been cloned and designated ETA and ETB (20-23). ETA and ETB belong to the large family of heptahelical G protein-coupled receptors. The ETA receptor shows a higher affinity for ET-1 than for ET-2 and lowest affinity for ET-3, while the ETB receptor shows approximately equal affinity for each of the three Endothelins (21, 22, 24). ETA is primarily responsible for the vasoconstrictor effects of ET-1 and is expressed by blood vessel smooth muscle cells (25, 26). The ETB receptor is also present in smooth muscle and the endothelia of blood vessels, kidney, lung, and brain (27). ET-1 has the ability to activate an array of signaling cascades including classical phosphatidylinositol turnover pathways leading to downstream PKC activation and Ca2+ mobilization (28-32). Other potential signaling mediators activated or produced by ET-1 include PI 3-kinase/Akt, NO, FAK, and Rho GTPases (32-37). ET-1 signaling may also be mediated indirectly via transactivation of the EGF receptor leading to downstream signaling by Ras and MAP kinases (38, 39). Injection of a single dose of ET-1 produces an initial decrease in systemic blood pressure followed by a prolonged increase in blood pressure (16, 40, 41). Blockade of Endothelin receptors with a systemic injection of an ETA/ETB antagonist causes progressive vasodilation, and elevated levels of ET-1 are found in some forms of human hypertension (42, 43). ET-1 also stimulates cardiac contraction and the growth of cardiac myocytes, regulates the release of vasoactive substances, and stimulates smooth muscle cell mitogenesis (32, 44-46). It also acts as a pro-survival factor for endothelial cells and regulates secretion by hypothalamic and pituitary cells (47, 48). ET-1 may control inflammatory responses by promoting the adhesion and migration of neutrophils and stimulating the production of pro-inflammatory cytokines (49-53). It has also been implicated in cancer progression at several levels including regulating the proliferation and migration of tumor cells and acting as a pro-angiogenic factor (54, 55). In addition, ET-1 has putative roles in other pathologies including septic shock, atherosclerosis, heart failure, renal insufficiency, pulmonary hypertension, and cerebrovascular conditions associated with subarachnoid hemorrhage (15, 56-63).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Endothelin-1 (DET100)(64)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.


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Human
(50)
Mouse
(6)
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Showing Publications 1 - 10 of 64. Show All 64 Publications.
Publications using DET100 Applications Species
S Macdonald, E Bosio, NI Shapiro, L Balmer, S Burrows, M Hibbs, T Jowitt, L Smart, G Arendts, D Fatovich No association between intravenous fluid volume and endothelial glycocalyx shedding in patients undergoing resuscitation for sepsis in the emergency department Scientific Reports, 2022;12(1):8733. 2022 [PMID: 35610344] (Human) Human
X Yang, R Dai, Z Qin, R Cai, Y Xu, Q Su LncRNA MALAT1 functions as a biomarker of no-reflow phenomenon in ST-segment elevation myocardial infarction patients receiving primary percutaneous coronary intervention Scientific Reports, 2022;12(1):3294. 2022 [PMID: 35228564] (Human) Human
V Montiel, I Lobysheva, L Gérard, M Vermeersch, D Perez-Morg, T Castelein, JB Mesland, P Hantson, C Collienne, D Gruson, MA van Dievoe, A Persu, C Beauloye, M Dechamps, L Belkhir, A Robert, M Derive, PF Laterre, AHJ Danser, X Wittebole, JL Balligand Oxidative stress-induced endothelial dysfunction and decreased vascular nitric oxide in COVID-19 patients EBioMedicine, 2022;77(0):103893. 2022 [PMID: 35219085] (Human) Human
CT Vong, Y Chen, Z Chen, C Gao, F Yang, S Wang, Y Wang Classical prescription Dachuanxiong Formula delays nitroglycerin-induced pain response in migraine mice through reducing endothelin-1 level and regulating fatty acid biosynthesis Journal of ethnopharmacology, 2022;0(0):114992. 2022 [PMID: 35032586] (Mouse) Mouse
X Nie, C Shen, J Tan, X Yang, W Wang, Y Dai, H Sun, Z Wu, J Chen Andrographolide Attenuates Established Pulmonary Hypertension via Rescue of Vascular Remodeling Biomolecules, 2021;11(12):. 2021 [PMID: 34944445] (Human) Human
M Velásquez, LF Peláez, M Rojas, R Narváez-Sá, JA Velásquez, C Escudero, S San Martín, ÁP Cadavid Differences in Endothelial Activation and Dysfunction Induced by Antiphospholipid Antibodies Among Groups of Patients With Thrombotic, Refractory, and Non-refractory Antiphospholipid Syndrome Frontiers in Physiology, 2021;12(0):764702. 2021 [PMID: 34925061] (Human) Human
I Dakota, M Munawar, R Pranata, WM Raffaello, R Sukmawan Diagnostic prediction model in subjects with low-risk unstable angina pectoris/non-ST segment Elevation Myocardial Infarction European review for medical and pharmacological sciences, 2021;25(16):5145-5152. 2021 [PMID: 34486689] (Human) Human
S Ríos-Arrab, JD Puentes-Pa, S Moreno-San, Á Szuba, J Casado, M García-Cos, J Escudero-F, M Verbeni, C Cano, C González-P, A Martín-Lag, Á Carazo, J León Endothelin-1 as a Mediator of Heme Oxygenase-1-Induced Stemness in Colorectal Cancer: Influence of p53 Journal of personalized medicine, 2021;11(6):. 2021 [PMID: 34199777] (Human) Human
HM Kang, M Hasanuzzam, SW Kim, HJ Koh, SC Lee Significant elevation of aqueous endothelin-1 in central retinal vein occlusion PLoS ONE, 2021;16(6):e0252530. 2021 [PMID: 34077461] (Human) Human
E Nassar, N Hassan, EA El-Ghonaim, H Hassan, MS Abdullah, TV Rottke, L Kiesel, B Greve, SA Ibrahim, M Götte Syndecan-1 Promotes Angiogenesis in Triple-Negative Breast Cancer through the Prognostically Relevant Tissue Factor Pathway and Additional Angiogenic Routes Cancers, 2021;13(10):. 2021 [PMID: 34066023] (Human) Human
Show All 64 Publications.

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