Endothelin-1 Antibody (3G10) [Unconjugated] - C-terminus

Endothelin-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human Endothelin-1 C-Terminus Monoclonal Antibody (Catalog # MAB3440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Recombinant Human Endothelin‑1 protein was serially diluted 2-fold and captured by Rat Anti-Human Endothelin‑1 C-Terminus Monoclonal Antibody (Catalog # MAB3440) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Endothelin‑1 C-Terminus Monoclonal Antibody (Catalog # MAB34401) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Double staining for CD31 and ET-1 in skin tissue fromthe amputated leg of CRPS patient B. CD31-positive blood vessels are stainedred and ET-1-positive cells are stained blue. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/18663383), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: ELISA, ICC/IF
• Clone: 3G10
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Endothelin-1 Antibody (3G10) [Unconjugated] - C-terminus Summary

• Immunogen: Human Endothelin-1
• Specificity: Detects human Endothelin‑1 C-Terminus. Recognizes the C-terminal region of mature Endothelin-1, -2, and -3 (amino acid residues 15‑21).
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: EDN1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Endothelin-1 Antibody (3G10) [Unconjugated] - C-terminus

• ARCND3
• EDN1
• endothelin 1
• Endothelin-1
• ET1
• HDLCQ7
• PPET1
• preproendothelin-1
• QME

Background

Endothelin-1, -2, and -3 are encoded by three separate genes as prepropolypeptides that are cleaved to yield inactive big endothelins. Big Endothelins are further cleaved to generate the 21 amino acid bioactive mature peptides. Endothelin-1 has potent vasoconstricting and angiogenic activity that is mediated through the 7TM receptors, ET-A and ET-B. Endothelin-1, -2, and -3 share identical amino acid sequences at their C-termini (residues 15‑21).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Endothelin-1 Antibody (MAB3440)(2)

Publications using MAB3440

• Perez A, Grodin J, Wu Y, Hernandez A, Butler J, Metra M, Felker G, Voors A, McMurray J, Armstrong P, Starling R, O'Connor C, Tang W Increased mortality with elevated plasma endothelin-1 in acute heart failure: an ASCEND-HF biomarker substudy. Eur J Heart Fail, 2015-12-14;18(3):290-7. 2015-12-14 [PMID: 26663359] (Human)
• Groeneweg JG, Antonissen CH, Huygen FJ Expression of endothelial nitric oxide synthase and endothelin-1 in skin tissue from amputated limbs of patients with complex regional pain syndrome. Mediators Inflamm., 2008-01-01;2008(0):680981. 2008-01-01 [PMID: 18663383] (IHC-Fr, Human)

Bioinformatics

• Gene Symbol: EDN1