Endothelin-1 Antibody (3D6) - Azide and BSA Free

Western Blot: Endothelin-1 Antibody (3D6) [H00001906-M01] - Analysis of EDN1 expression in transfected 293T cell line by EDN1 monoclonal antibody (M01), clone 3D6.Lane 1: EDN1 transfected lysate(24 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: Endothelin-1 Antibody (3D6) [H00001906-M01] - Analysis of EDN1 transfected lysate using anti-EDN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EDN1 MaxPab rabbit polyclonal antibody.

Sandwich ELISA: Endothelin-1 Antibody (3D6) [H00001906-M01] - Detection limit for recombinant GST tagged EDN1 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IP
• Clone: 3D6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Endothelin-1 Antibody (3D6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: EDN1 (AAH09720, 113 a.a. ~ 212 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SQKDKKCWNFCQAGKELRAEDIMEKDWNNHKKGKDCSKLGKKCIYQQLVRGRKIRRSSEEHLRQTRSETMRNSVKSSFHDPKLKGNPSRERYVTHNRAHW
• Specificity: EDN1 - endothelin 1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EDN1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Endothelin-1 Antibody (3D6) - Azide and BSA Free

• ARCND3
• EDN1
• endothelin 1
• Endothelin-1
• ET1
• HDLCQ7
• PPET1
• preproendothelin-1
• QME

Background

Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Endothelin-1 Antibody (H00001906-M01)(4)

Publications using H00001906-M01

• Ramshani Z, Fan F, Wei A et al. A multiplexed immuno-sensor for on-line and automated monitoring of tissue culture protein biomarkers Talanta 2021-02-17 [PMID: 33592751]
• Marion A, Dieudonne FX, Patino-Garcia A et al. Calpain-6 is an endothelin-1 signaling dependent protective factor in chemoresistant osteosarcoma. Int J Cancer. 2011-08-16 [PMID: 21681744]
• Schildroth J, Rettig-Zimmermann J, Kalk P et al. dothelin type A and B receptors in the control of afferent and efferent arterioles in mice. Nephrol Dial Transplant. 2010-09-02 [PMID: 20813769]
• Ghoul A, Serova M, Astorgues-Xerri L et al. Epithelial-to-Mesenchymal Transition and Resistance to Ingenol 3-Angelate, a Novel Protein Kinase C Modulator, in Colon Cancer Cells. Cancer Res. 2009-05-15 [PMID: 19417139]

Bioinformatics

• Gene Symbol: EDN1
• Entrez:
  • 1906(Human)
• OMIM:
  • 131240(Human)
• Uniprot:
  • AAH09720(Human)