Endothelial Lipase Antibody (4A9) - Azide and BSA Free

Western Blot: Endothelial Lipase Antibody (4A9) [H00009388-M01] - Analysis of LIPG expression in transfected 293T cell line by LIPG monoclonal antibody (M01), clone 4A9.Lane 1: LIPG transfected lysate (Predicted MW: 56.8 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: Endothelial Lipase Antibody (4A9) [H00009388-M01] - Analysis of LIPG transfected lysate using anti-LIPG monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LIPG MaxPab rabbit polyclonal antibody.

ELISA: Endothelial Lipase Antibody (4A9) [H00009388-M01] - Detection limit for recombinant GST tagged LIPG is 3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 4A9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Endothelial Lipase Antibody (4A9) - Azide and BSA Free Summary

• Immunogen: LIPG (AAH60825.1, 21 a.a. ~ 500 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SPVPFGPEGRLEDKLHKPKATQTEVKPSVRFNLRTSKDPEHEGCYLSVGHSQPLEDCSFNMTAKTFFIIHGWTMSGIFENWLHKLVSALHTREKDANVVVVDWLPLAHQLYTDAVNNTRVVGHSIARMLDWLQEKDDFSLGNVHLIGYSLGAHVAGYAGNFVKGTVGRITGLDPAGPMFEGADIHKRLSPDDADFVDVLHTYTRSFGLSIGIQMPVGHIDIYPNGGDFQPGCGLNDVLGSIAYGTITEVVKCEHERAVHLFVDSLVNQDKPSFAFQCTDSNRFKKGICLSCRKNRCNSIGYNAKKMRNKRNSKMYLKTRAGMPFRVYHYQMKIHVFSYKNMGEIEPTFYVTLYGTNADSQTLPLEIVERIEQNATNTFLVYTEEDLGDLLKIQLTWEGASQSWYNLWKEFRSYLSQPRNPGRELNIRRIRVKSGETQRKLTFCTEDPENTSISPGQELWFRKCRDGWRMKNETSPTVELP
• Specificity: LIPG - lipase, endothelial (4A9)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LIPG
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Endothelial Lipase Antibody (4A9) - Azide and BSA Free

• EC 3.1.1
• EC 3.1.1.3
• EDLendothelial lipase
• ELlipoprotein lipase H
• Endothelial cell-derived lipase
• lipase, endothelial
• PRO719

Background

The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LIPG
• Entrez:
  • 9388(Human)
• Uniprot:
  • AAH60825(Human)