Enah/Vasp-like Antibody (5G1) - Azide and BSA Free

Western Blot: Enah/Vasp-like Antibody (5G1) [H00051466-M01] - Analysis of EVL expression in transfected 293T cell line by EVL monoclonal antibody (M01), clone 5G1.Lane 1: EVL transfected lysate(45 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Enah/Vasp-like Antibody (5G1) [H00051466-M01] - Analysis of monoclonal antibody to EVL on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: Enah/Vasp-like Antibody (5G1) [H00051466-M01] - Detection limit for recombinant GST tagged EVL is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 5G1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Enah/Vasp-like Antibody (5G1) - Azide and BSA Free Summary

• Immunogen: EVL (NP_057421, 309 a.a. ~ 418 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PGTRAASQPPNSSEAGRKPWERSNSVEKPVSSILSRTPSVAKSPEAKSPLQSQPHSRMKPAGSVNDMALDAFDLDRMKQEILEEVVRELHKVKEEIIDAIRQELSGISTT
• Localization: Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Note=Targeted to the leading edge of lamellipodia and the distal tip of stress fibers through interaction with a number of proteins. In activated T-cells, localizes to the F-actin collar and the dis
• Specificity: EVL - Enah/Vasp-like
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EVL
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA immunofluorescence.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Enah/Vasp-like Antibody (5G1) - Azide and BSA Free

• ena/VASP-like protein
• Enah/Vasp-like
• RNB6Ena/vasodilator-stimulated phosphoprotein-like

Background

Enah/Vasp-like (EVL) is a member of the Ena/VASP protein family. Ena/VASP proteins are actin-associated proteins involved in a range of processes that require dynamic actin remodeling such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EVL
• Entrez:
  • 51466(Human)
• Uniprot:
  • NP_057421(Human)