EN1/Engrailed 1 Antibody

Immunohistochemistry-Paraffin: EN1/Engrailed 1 Antibody [NBP2-57757] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Immunohistochemistry-Paraffin: EN1/Engrailed 1 Antibody [NBP2-57757] - Staining of human skin shows strong nuclear positivity in sweat glands.

Immunohistochemistry-Paraffin: EN1/Engrailed 1 Antibody [NBP2-57757] - Staining of human cervix, uterine shows no postivity in squamous epithelial cells as expected.

Immunohistochemistry-Paraffin: EN1/Engrailed 1 Antibody [NBP2-57757] - Staining of human kidney shows no positivity in cells in tubules as expected.

Product Discontinued

EN1/Engrailed 1 Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: GAQGTKYPEHGNPAILLMGSANG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EN1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Mouse 87%, Rat 87%

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for EN1/Engrailed 1 Antibody

• engrailed homeobox 1
• engrailed homolog 1
• Homeobox protein en-1
• homeobox protein engrailed-1
• hu-En-1

Background

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EN1/Engrailed 1 Antibody (NBP2-57757)(1)

Publication using NBP2-57757

• Gyorfi Ah, Matei Ae, Fuchs M Et Al. Engrailed 1 coordinates cytoskeletal reorganization to induce myofibroblast differentiation The Journal of experimental medicine 2021-09-06 [PMID: 34259830]

Bioinformatics

• Gene Symbol: EN1