EMMPRIN/CD147 Antibody [Unconjugated]

Western blot shows lysates of mouse brain tissue, mouse heart tissue, and mouse small intestine tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF772) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EMMPRIN/CD147 at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of mouse brain (cortex) tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for EMMPRIN/CD147 at approximately 62 kDa (as indicated) using 2 µg/mL of Goat Anti-Mouse EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF772) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Mu
• Applications: WB, Simple Western, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

EMMPRIN/CD147 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse EMMPRIN/CD147
  Ala22-Arg209
  Accession # NP_001070652
• Specificity: Detects mouse EMMPRIN/CD147 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human EMMPRIN is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BSG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EMMPRIN/CD147 Antibody [Unconjugated]

• 5F7
• basigin (Ok blood group)
• Basigin
• BSG
• CD147 antigen
• CD147
• Collagenase stimulatory factor
• EMMPRIN
• EMMPRINTCSF
• Extracellular matrix metalloproteinase inducer
• Leukocyte activation antigen M6
• M6
• OK blood group antigen
• OK
• Tumor cell-derived collagenase stimulatory factor

Background

EMMPRIN, also known as basigin and CD147, is a type I membrane protein belonging to the immunoglobulin superfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EMMPRIN/CD147 Antibody (AF772)(11)

Publications using AF772

• G Feigelman, E Simanovich, P Brockmeyer, MA Rahat Knocking-Down CD147/EMMPRIN Expression in CT26 Colon Carcinoma Forces the Cells into Cellular and Angiogenic Dormancy That Can Be Reversed by Interactions with Macrophages Biomedicines, 2023-03-02;11(3):. 2023-03-02 [PMID: 36979746] (IF, Western Blot, Mouse)
• Z Benyahia, MCNM Blackman, L Hamelin, LX Zampieri, T Capeloa, ML Bedin, T Vazeille, O Schakman, P Sonveaux In Vitro and In Vivo Characterization of MCT1 Inhibitor AZD3965 Confirms Preclinical Safety Compatible with Breast Cancer Treatment Cancers, 2021-02-02;13(3):. 2021-02-02 [PMID: 33540599] (Western Blot, Mouse)
• Lv JJ, Wang H, Cui HY, et al. Blockade of Macrophage CD147 Protects Against Foam Cell Formation in Atherosclerosis Frontiers in cell and developmental biology 1/8/2020 [PMID: 33490072]
• Tian Zhang, Hao Li, Ke Wang, Bing Xu, Zhi-Nan Chen, Huijie Bian Deficiency of CD147 Attenuated Non-alcoholic Steatohepatitis Progression in an NLRP3-Dependent Manner Frontiers in Cell and Developmental Biology 8/13/2020 [PMID: 32903542]
• Tian Zhang, Hao Li, Ke Wang, Bing Xu, Zhi-Nan Chen, Huijie Bian Deficiency of CD147 Attenuated Non-alcoholic Steatohepatitis Progression in an NLRP3-Dependent Manner Frontiers in Cell and Developmental Biology 2020-08-13 [PMID: 32903542] (Western Blot, Mouse)
• Souvik Dey, Alaa Eisa, Douglas Kline, Florence F. Wagner, Sanjaya Abeysirigunawardena, Srinivasan Vijayaraghavan Roles of glycogen synthase kinase 3 alpha and calcineurin in regulating the ability of sperm to fertilize eggs The FASEB Journal 1/1/2020 [PMID: 31914663]
• Mohammad Abdul Hai Siddique, Kimio Satoh, Ryo Kurosawa, Nobuhiro Kikuchi, Md. Elias-Al-Mamun, Junichi Omura et al. Identification of Emetine as a Therapeutic Agent for Pulmonary Arterial Hypertension: Novel Effects of an Old Drug Arteriosclerosis, Thrombosis, and Vascular Biology 11/1/2019 [PMID: 31533472]
• Kato N, Kosugi T, Sato W, Ishimoto T, Kojima H, Sato Y, Sakamoto K, Maruyama S, Yuzawa Y, Matsuo S, Kadomatsu K Basigin/CD147 promotes renal fibrosis after unilateral ureteral obstruction. Am. J. Pathol., 2011-02-01;178(2):572-9. 2011-02-01 [PMID: 21281789] (IHC-P, Western Blot, Mouse)
• Kato N, Yuzawa Y, Kosugi T The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion. J. Am. Soc. Nephrol., 2009-05-14;20(7):1565-76. 2009-05-14 [PMID: 19443639] (Western Blot, Mouse)
• Chen L, Belton RJ, Nowak RA Basigin-mediated gene expression changes in mouse uterine stromal cells during implantation. Endocrinology, 2008-10-01;150(2):966-76. 2008-10-01 [PMID: 18832103] (IHC-P, Mouse)
• Ramo K, Cashman SM, Kumar-Singh R Evaluation of adenovirus-delivered human CD59 as a potential therapy for AMD in a model of human membrane attack complex formation on murine RPE. Invest. Ophthalmol. Vis. Sci., 2008-05-16;49(9):4126-36. 2008-05-16 [PMID: 18487376] (ICC, IHC, Mouse)

Bioinformatics

• Gene Symbol: BSG
• Uniprot:
  • NP_001070652()