EMMPRIN/CD147 Antibody (OX114)

Images

 
Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) [NB100-65518] - Analysis using the FITC conjugate of NB100-65518. Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD147: FITC.
Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) [NB100-65518] - Staining of mouse peripheral blood lymphocytes with RAT ANTI MOUSE CD147.
Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) [NB100-65518] - Staining of mouse peripheral blood lymphocytes with Rat anti MouseE CD147: FITC.
Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) [NB100-65518] - Staining of mouse spleen cells with Rat anti Mouse CD147: Alexa Fluor 488.
Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) [NB100-65518] - Analysis using the Biotin conjugate of NB100-65518. Staining of mouse spleen cells with RAT ANTI MOUSE CD147: BIOTIN.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr
Clone
OX114
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

EMMPRIN/CD147 Antibody (OX114) Summary

Immunogen
Fusion protein mCD147dO-2rCD4d3 +4
Specificity
NB100-65518 recognizes mouse CD147 (also known as basigin). CD147 is a cell surface glycoprotein that is widely expressed on leukocytes, red cells, platelets and endothelial cells. The reported functions of CD147 are diverse and include involvement in T-cell activation, cell adhesion and induction of matrix metalloproteinases. OX-114 recognizes both isoforms of CD147, the common form containing 2 Ig-like domains and the rare form containing 3 Ig-like domains. This antibody is routinely tested in flow cytometry on mouse splenocytes. Note that for staining of whole blood samples increased amounts of antibody may be required due to the presence of CD147 on erythrocytes.
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
BSG
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:25-1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for EMMPRIN/CD147 Antibody (OX114)

  • 5F7
  • basigin (Ok blood group)
  • Basigin
  • BSG
  • CD147 antigen
  • CD147
  • Collagenase stimulatory factor
  • EMMPRIN
  • EMMPRINTCSF
  • Extracellular matrix metalloproteinase inducer
  • Leukocyte activation antigen M6
  • M6
  • OK blood group antigen
  • OK
  • Tumor cell-derived collagenase stimulatory factor

Background

CD147 plays a pivotal role in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. CD147 also seems to be a receptor for oligomannosidic glycans. In vitro, it promotes outgrowth of astrocytic processes. CD147 forms homooligomers in a cis-dependent manner on the plasma membrane. It forms a complex with MMP1 at the tumor cell surface. CD147 interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. CD147 also interacts with ATP1B2, MAG and L1CAM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol BSG
Entrez