EMMPRIN/CD147 Antibody (MEM-M6/2) [FITC]

Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/2) [FITC] [NBP1-44585] - Surface staining of human peripheral blood with anti-human CD147 (MEM-M6/1) FITC.

Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/2) [FITC] [NBP1-44585] - Surface staining of human peripheral blood with anti-human CD147 (MEM-M6/1) biotin.

• Reactivity: Hu, Po
• Applications: Flow
• Clone: MEM-M6/2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

EMMPRIN/CD147 Antibody (MEM-M6/2) [FITC] Summary

• Description: Store in the dark. Avoid prolonged exposure to light.
• Immunogen: A soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the entire extracellular region of CD147 fused to the DNA coding for the hinge region, CH2 and CH3 domain of human IgG1.
• Epitope: This antibody recognises extracellular Ig domain D1 of CD147 antigen.
• Localization: Cell Membrane
• Specificity: The antibody MEM-M6/2 recognizes extracellular Ig domain D1 of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes. The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: BSG
• Purity: Size Exclusion Chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
• Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Purity: Size Exclusion Chromatography

Alternate Names for EMMPRIN/CD147 Antibody (MEM-M6/2) [FITC]

• 5F7
• basigin (Ok blood group)
• Basigin
• BSG
• CD147 antigen
• CD147
• Collagenase stimulatory factor
• EMMPRIN
• EMMPRINTCSF
• Extracellular matrix metalloproteinase inducer
• Leukocyte activation antigen M6
• M6
• OK blood group antigen
• OK
• Tumor cell-derived collagenase stimulatory factor

Background

CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumour cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BSG