EMAP-II/AIMP1 Antibody

Western Blot: EMAP-II/AIMP1 Antibody [NBP2-26124] - NBP2-26124 (0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

EMAP-II/AIMP1 Antibody Summary

• Immunogen: Peptide with sequence NNDAVLKRLEQK-C corresponding to N-Terminus according to NP_001135888.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_004748.2; NP_001135888.1). Reported variants represent identical protein: NP_004748.2, NP_001135887.1.
• Predicted Species: Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AIMP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 37 kDa band observed in lysates of HeLa and Jurkat, and in lysates of mouse and rat spleen (calculated MW of 37 kDa band according to NP_001135887.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EMAP-II/AIMP1 Antibody

• AIMP1
• aminoacyl tRNA synthetase complex-interacting multifunctional protein 1
• EMAP-2
• EMAPII
• EMAP-II
• EMAPIImember 1 (endothelialmonocyte-activating)
• endothelial-monocyte activating polypeptide II
• Multisynthase complex auxiliary component p43
• multisynthetase complex auxiliary component p43
• SCYE1

Background

EMAP II (Endothelial-Monocyte-Activating Polypeptide II) is a proinflammatory cytokine and chemoattractant of macrophages and polymorphonuclear lymphocytes. EMAP II is synthesized as a 34 kDa precursor molecule which is cleaved to a biologically active 22 kDa mature polypeptide. This active protein is known to induce the procoagulant protein tissue factor on the surface of endothelial cells and modulate other properties of endothelial cells and monocytes in vitro, including the stimulation of TNF-alpha and myeloperoxidase. EMAP II activates neutrophils in vivo and induces an inflammatory reaction and tumor regression. EMAP II induces apoptosis in proliferating endothelial cells and inhibits neovascularization. This anti-angiogenic property is of interest to tumor biology. EMAP II is also a negative modulator of lung vascular growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EMAP-II/AIMP1 Antibody (NBP2-26124)(1)

Publication using NBP2-26124

• Kim SS, Hur SY, Kim YR et al. Expression of AIMP1, 2 and 3, the scaffolds for the multi-tRNA synthetase complex, is downregulated in gastric and colorectal cancer. Tumori 2011-05-01 [PMID: 21789020]

Bioinformatics

• Gene Symbol: AIMP1
• Entrez:
  • 9255(Human)
  • 13722(Mouse)
  • 114632(Rat)