ELOVL3 Overexpression Lysate (Native)

Images

 
Western Blot: ELOVL3 Overexpression Lysate (Adult Normal) [NBP2-05402] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ELOVL3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ELOVL3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_689523. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ELOVL3, mRNA.
Gene
ELOVL3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
31.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ELOVL3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ELOVL3 Overexpression Lysate (Native)

  • 3-keto acyl-CoA synthase ELOVL3
  • CIG-30
  • CIG30MGC21435
  • Cold-inducible glycoprotein of 30 kDa
  • EC 2.3.1.n8
  • elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 3
  • elongation of very long chain fatty acids protein 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB600-582
Species: Hu, Mu, Rt, Ca, Ch, SyHa, Ha
Applications: WB, Simple Western
NBP2-22106
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB300-537
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP
NBP1-92274
Species: Hu
Applications: WB, IHC, IHC-P
NB400-114
Species: Hu, Mu, Rt, Po, Ch, Ha, Pl, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB100-150
Species: Hu, Mu
Applications: WB, ICC/IF, IP
NBP1-82082
Species: Hu
Applications: IHC, IHC-P
NBP1-86960
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
H00253782-A01
Species: Hu, Mu
Applications: WB, ELISA
NB400-157
Species: Hu, Mu, Po, Pm
Applications: WB, Simple Western
NBP2-37956
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB110-40760
Species: Hu, Mu, Rt, Po
Applications: WB, ICC/IF, IHC, IHC-P
H00054898-P01
Species: Hu
NBP1-88644
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00005308-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
AF1443
Species: Mu, Rt
Applications: WB, Simple Western, IHC, ICC
NBP2-23450
Species: Mu
NBP1-89269
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF6574
Species: Mu
Applications: WB
NBP2-05402
Species: Hu
Applications: WB

Publications for ELOVL3 Lysate (NBP2-05402) (0)

There are no publications for ELOVL3 Lysate (NBP2-05402).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ELOVL3 Lysate (NBP2-05402) (0)

There are no reviews for ELOVL3 Lysate (NBP2-05402). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ELOVL3 Lysate (NBP2-05402) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ELOVL3 Products

Bioinformatics Tool for ELOVL3 Lysate (NBP2-05402)

Discover related pathways, diseases and genes to ELOVL3 Lysate (NBP2-05402). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ELOVL3 Lysate (NBP2-05402)

Discover more about diseases related to ELOVL3 Lysate (NBP2-05402).
 

Pathways for ELOVL3 Lysate (NBP2-05402)

View related products by pathway.

PTMs for ELOVL3 Lysate (NBP2-05402)

Learn more about PTMs related to ELOVL3 Lysate (NBP2-05402).

Blogs on ELOVL3

There are no specific blogs for ELOVL3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

ELOVL3 Antibody
NBP1-85618

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ELOVL3 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol ELOVL3