ELMO1 Antibody

Western Blot: ELMO1 Antibody [NB100-875] - (0.3ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunoprecipitation: ELMO1 Antibody [NB100-875] - (0.3ug/ml) staining of Human Frontal Cortex (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ze
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ELMO1 Antibody Summary

• Description: Novus Biologicals Goat ELMO1 Antibody (NB100-875) is a polyclonal antibody validated for use in WB, ELISA and IP. Anti-ELMO1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence PKEPSNYDFVYDCN corresponding to C-Terminus according to NP_055615.8, NP_569709.1, NP_001034548.1.
• Specificity: This antibody is expected to recognise both human isoforms of this protein. NP_569709.1 and NP_001034548.1 represent the same isoform.
• Predicted Species: Canine (100%), Bovine (100%), Zebrafish (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ELMO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10 - 1:500
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: Western blot: Approx 83kDa band observed in Human Brain (Frontal Cortex) lysates, in Mouse and Rat Brain lysates and in lysates of cell line Jurkat (calculated MW of 83.8kDa according to NP_055615.8).
  Immunoprecipitation: This antibody has been successfully used in IP from BAEC cells as described in the following paper: Epting et al, Circ Res. 2010 Jul 9;107(1):45-55; PMID: 20466982. IP; An anonymous customer found positive results in IP on Human promyelocytic leukemia cells (HL60)
• Control:

  ELMO1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ELMO1 Antibody

• ced-12 homolog 1
• CED12CED-12
• ELMO-1
• engulfment and cell motility 1 (ced-12 homolog, C. elegans)
• engulfment and cell motility 1
• engulfment and cell motility protein 1
• KIAA0281MGC126406
• Protein ced-12 homolog

Background

The protein encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein to promote phagocytosis and effect cell shape changes. Similarity to a C. elegans protein suggests that this protein may function in apoptosis and in cell migration. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ELMO1 Antibody (NB100-875)(2)

Publications using NB100-875

• Epting D, Wendik B, Bennewitz K et al. The Rac1 regulator ELMO1 controls vascular morphogenesis in zebrafish. Circ Res 2010-07-01 [PMID: 20466982]
• Gumienny TL, Brugnera E, Tosello-Trampont AC et al. CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration. Cell 2001-10-05 [PMID: 11595183]

Bioinformatics

• Gene Symbol: ELMO1
• Entrez:
  • 9844(Human)
  • 140580(Mouse)
• Uniprot:
  • NP_055615(Human)