ELMO1 Antibody

Images

 
Western Blot: ELMO1 Antibody [NB100-875] - (0.3ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunoprecipitation: ELMO1 Antibody [NB100-875] - (0.3ug/ml) staining of Human Frontal Cortex (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IP, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ELMO1 Antibody Summary

Immunogen
Peptide with sequence PKEPSNYDFVYDCN corresponding to C-Terminus according to NP_055615.8, NP_569709.1, NP_001034548.1.
Specificity
This antibody is expected to recognise both human isoforms of this protein. NP_569709.1 and NP_001034548.1 represent the same isoform.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ELMO1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunoprecipitation 1:10 - 1:500
  • Peptide ELISA Detection limit 1:128000
Application Notes
Western blot: Approx 83kDa band observed in Human Brain (Frontal Cortex) lysates, in Mouse and Rat Brain lysates and in lysates of cell line Jurkat (calculated MW of 83.8kDa according to NP_055615.8).
Immunoprecipitation: This antibody has been successfully used in IP from BAEC cells as described in the following paper: Epting et al, Circ Res. 2010 Jul 9;107(1):45-55; PMID: 20466982. IP; An anonymous customer found positive results in IP on Human promyelocytic leukemia cells (HL60)
Control
ELMO1 Lysate (NBL1-10239)
Publications
Read Publications using NB100-875.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine and Zebra Fish

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ELMO1 Antibody

  • ced-12 homolog 1
  • CED12CED-12
  • ELMO-1
  • engulfment and cell motility 1 (ced-12 homolog, C. elegans)
  • engulfment and cell motility 1
  • engulfment and cell motility protein 1
  • KIAA0281MGC126406
  • Protein ced-12 homolog

Background

The protein encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein to promote phagocytosis and effect cell shape changes. Similarity to a C. elegans protein suggests that this protein may function in apoptosis and in cell migration. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ELMO1 Antibody (NB100-875)(2)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ELMO1
Entrez
Uniprot