ELMO1 Overexpression Lysate (Native)

Images

 
Western Blot: ELMO1 Overexpression Lysate (Adult Normal) [NBL1-10239] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ELMO1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ELMO1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC221438 and based on accession number NM_130442. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens engulfment and cell motility 1 (ELMO1), transcript variant 2, mRNA.
Gene
ELMO1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
28.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ELMO1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ELMO1 Overexpression Lysate (Native)

  • ced-12 homolog 1
  • CED12CED-12
  • ELMO-1
  • engulfment and cell motility 1 (ced-12 homolog, C. elegans)
  • engulfment and cell motility 1
  • engulfment and cell motility protein 1
  • KIAA0281MGC126406
  • Protein ced-12 homolog

Background

The protein encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein to promote phagocytosis and effect cell shape changes. Similarity to a C. elegans protein suggests that this protein may function in apoptosis and in cell migration. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq]. Transcript Variant: This variant (2) includes a unique exon in its 5' end, resulting in use of a downstream in-frame start codon as compared to variant 1. Isoform 2 is 480 aa shorter than isoform 1. Transcript variants 2 and 3 encoded the same protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for ELMO1 Lysate (NBL1-10239) (0)

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Video Protocols

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Bioinformatics

Gene Symbol ELMO1