eIF5A Antibody (SC60-09)

Western Blot: eIF5A Antibody (SC60-09) [NBP2-67591] - Western blot analysis of eIF5A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab

Immunocytochemistry/Immunofluorescence: eIF5A Antibody (SC60-09) [NBP2-67591] - Staining eIF5A in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: eIF5A Antibody (SC60-09) [NBP2-67591] - Analysis of paraffin-embedded mouse liver tissue using anti-eIF5A antibody. Counter stained with hematoxylin.

Flow Cytometry: eIF5A Antibody (SC60-09) [NBP2-67591] - Analysis of Jurkat cells with eIF5A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: eIF5A Antibody (SC60-09) [NBP2-67591] - Analysis of eIF5A on different lysates using anti-eIF5A antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: MCF-7

Immunocytochemistry/Immunofluorescence: eIF5A Antibody (SC60-09) [NBP2-67591] - Staining eIF5A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: eIF5A Antibody (SC60-09) [NBP2-67591] - Analysis of paraffin-embedded human kidney tissue using anti-eIF5A antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: eIF5A Antibody (SC60-09) [NBP2-67591] - Analysis of paraffin-embedded mouse kidney tissue using anti-eIF5A antibody. Counter stained with hematoxylin.

Product Discontinued

eIF5A Antibody (SC60-09) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within N-terminal human eIF5A (SwissProt: P63241 Human; SwissProt: P63242 Mouse; SwissProt: Q3T1J1 Rat)
• Localization: Cytoplasm, Nucleus, Endoplasmic reticulum membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EIF5A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for eIF5A Antibody (SC60-09)

• eIF4D
• eIF-4D
• eIF5A
• EIF-5A
• EIF5A1
• eIF-5A1
• eIF-5A-1
• eIF5AI
• Eukaryotic initiation factor 5A isoform 1
• eukaryotic initiation factor 5A
• eukaryotic translation initiation factor 5A
• eukaryotic translation initiation factor 5A-1
• MGC104255
• MGC99547
• Rev-binding Factor

Background

The human eukaryotic translation initiation factor 5A (eIF5A) is an 18 kDa protein. eIF5A precursor [(ec-eIF-5A(lys)] is the only cellular protein known to contain a specific lysine residue which is transformed into the unique amino acid hypusine [N-(4-amino-2-hydroxybutyl)-lysine] by a series of post translational reactions. eIF5A promotes the formation of the first peptide bond during the initial stage of protein synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF5A