eIF5A Antibody (SC60-09)

Images

 
Western Blot: eIF5A Antibody (SC60-09) [NBP2-67591] - Western blot analysis of eIF5A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The ...read more
Immunocytochemistry/ Immunofluorescence: eIF5A Antibody (SC60-09) [NBP2-67591] - Staining eIF5A in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: eIF5A Antibody (SC60-09) [NBP2-67591] - Analysis of paraffin-embedded mouse liver tissue using anti-eIF5A antibody. Counter stained with hematoxylin.
Flow Cytometry: eIF5A Antibody (SC60-09) [NBP2-67591] - Analysis of Jurkat cells with eIF5A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). ...read more
Western Blot: eIF5A Antibody (SC60-09) [NBP2-67591] - Analysis of eIF5A on different lysates using anti-eIF5A antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: MCF-7
Immunocytochemistry/ Immunofluorescence: eIF5A Antibody (SC60-09) [NBP2-67591] - Staining eIF5A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: eIF5A Antibody (SC60-09) [NBP2-67591] - Analysis of paraffin-embedded human kidney tissue using anti-eIF5A antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: eIF5A Antibody (SC60-09) [NBP2-67591] - Analysis of paraffin-embedded mouse kidney tissue using anti-eIF5A antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
SC60-09
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

eIF5A Antibody (SC60-09) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within N-terminal human eIF5A (SwissProt: P63241 Human; SwissProt: P63242 Mouse; SwissProt: Q3T1J1 Rat)
Localization
Cytoplasm, Nucleus, Endoplasmic reticulum membrane.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
EIF5A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for eIF5A Antibody (SC60-09)

  • eIF4D
  • eIF-4D
  • eIF5A
  • EIF-5A
  • EIF5A1
  • eIF-5A1
  • eIF-5A-1
  • eIF5AI
  • Eukaryotic initiation factor 5A isoform 1
  • eukaryotic initiation factor 5A
  • eukaryotic translation initiation factor 5A
  • eukaryotic translation initiation factor 5A-1
  • MGC104255
  • MGC99547
  • Rev-binding Factor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol EIF5A