eIF4B Antibody

Western blot shows lysates of A431 human epithelial carcinoma cell line and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human eIF4B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3800) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eIF4B at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

eIF4B Antibody Summary

• Immunogen: E. coli-derived recombinant human eIF4B
  Met1-Arg200
  Accession # P23588
• Specificity: Detects human and mouse eIF4B in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EIF4B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for eIF4B Antibody

• Eif4a2
• eIF4B
• EIF-4B
• eukaryotic translation initiation factor 4B
• PRO1843

Background

eIF4B (eukaryotic translation initiation factor 4B),an RNA binding protein, is essential for the binding of mRNA to the 43S pre-initiation complex, which consists of the 40S ribosomal subunit bound to a ternary complex of eIF2, GTP, and Met-tRNA stabilized by eIF3. An arginine rich motif (ARM) in the carboxy-terminus of eIF4B binds RNA non-specifically, while a canonical RNA motif (RMM) near the amino terminus binds specifically to 18S rRNA. The simultaneous binding of specific and nonspecific RNA may serve to facilitate the binding of the 40S subunit to the mRNA by serving as a bridge between the 18S rRNA and the mRNA. Besides its binding activity, eIF4B in conjunction with eIF4F, a heterotrimeric protein, stimulates the ATPase and RNA helicase activity of eIF4E.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF4B
• Uniprot:
  • P23588()