eIF4A2 Antibody

Western Blot: eIF4A2 Antibody [NBP2-24612] - Analysis of eIF4A2 in human liver lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse liver lysate using NBP2-24612 at 0.5 ug/ml.

Immunohistochemistry-Paraffin: eIF4A2 Antibody [NBP2-24612] - Analysis of eIF4A2 in human brain tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.

Product Discontinued

eIF4A2 Antibody Summary

• Immunogen: A portion of amino acids 1-50 of human eIF4A2 was used as the immunogen.
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF4A2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.5-1.0 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for eIF4A2 Antibody

• ATP-dependent RNA helicase eIF4A-2
• BM-010
• DDX2Beukaryotic translation initiation factor 4A, isoform 2
• EC 3.6.1
• EC 3.6.4.13
• EIF4A
• eIF-4A-II
• eIF4A-II
• EIF4F
• eukaryotic initiation factor 4A-II
• eukaryotic translation initiation factor 4A
• eukaryotic translation initiation factor 4A2

Background

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and isrequired for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondarystructures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, andsubsequent scanning for the initiator codon

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for eIF4A2 Antibody (NBP2-24612)(1)

Publication using NBP2-24612

• Liu L, Vujovic A, Deshpande NP et al. The splicing factor RBM17 drives leukemic stem cell maintenance by evading nonsense-mediated decay of pro-leukemic factors Nature communications 2022-07-04 [PMID: 35781533] (WB, Human)
  
  Details:
  Dilution: 1:1000

FAQs for eIF4A2 Antibody (NBP2-24612). (Showing 1 - 2 of 2 FAQ).

1. I am looking for an antibody that reacts specifically with eIF4A2 (and not eIF4A1, which is extremely similar) and that works in IP. You sell an antibody (NBP1-57490) but based on the information provided I am not able to decide if the antibody meets my requirements. Do you know if it does? If not I do have overexpression plasmids for tagged eIF4A1 and eIF4A2 that would allow me to test it for you if you could provide with a little of the antibody.
   • The immunogens for NBP2-24612 and NBP1-57490 share under 75% similarity with eIF4A2 and are not predicted to cross-react. They have shown no cross-reactivity in testing and should suit your needs.
2. How has the cross-reactivity been tested? Do the antibodies work in IP?
   • The immunogens are carefully chosen to minimize the chance of cross-reactivity with other proteins. None of our testing or customer feedback has suggested that either NBP2-24612 or NBP1-57490 cross-react. NBP2-24612 has been validated in Western blot and in IHC on paraffin-embedded tissues. NBP1-57490 has been validated in Western blot only. They have never been tested for use in IP. If you wanted to chose one to try to use in IP, I would recommend NBP2-24612, as it works in IHC-P as well, so it is known to recognize the protein in its native form.

Bioinformatics

• Gene Symbol: EIF4A2
• Uniprot:
  • Q14240(Human)