Recombinant Human eIF4A1 Protein

Product Discontinued

Recombinant Human eIF4A1 Protein Summary

• Description: Human EIF4A1 (NP_001407.1) full-length recombinant protein expressed in yeast. Sequence: MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Yeast
• Protein/Peptide Type: Recombinant Protein
• Gene: EIF4A1
• Purity: >90% SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 46.101 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 50 mM HEPES, 100mM NaCl, pH 7.5 in 30% glycerol, 30 mM Glutathione, 0.5% TritonX-100, 1 mM dithiothreitol.
• Preservative: No Preservative
• Purity: >90% SDS-PAGE

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human eIF4A1 Protein

• ATP-dependent RNA helicase eIF4A-1
• DDX2Aeukaryotic initiation factor 4A-I
• EC 3.6.1
• EC 3.6.4.13
• EIF4A
• EIF-4A
• eIF-4A-I
• eIF4A-I
• eukaryotic initiation factor 4AI
• eukaryotic translation initiation factor 4A
• eukaryotic translation initiation factor 4A, isoform 1
• eukaryotic translation initiation factor 4A1

Background

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and isrequired for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondarystructures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, andsubsequent scanning for the initiator codon

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: EIF4A1