EIF5 Antibody

Images

 
Western Blot: EIF5 Antibody [NBP2-16311] - EIF5 antibody detects EIF5 protein by Western blot analysis. A. 50 ug mouse brain lysate/extract 10 % SDS-PAGE EIF5 antibody dilution: 1:1000
Immunohistochemistry-Paraffin: EIF5 Antibody [NBP2-16311] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.
Western Blot: EIF5 Antibody [NBP2-16311] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: EIF5 Antibody [NBP2-16311] - EIF5 antibody detects EIF5 protein by Western blot analysis. A. 50 ug rat brain lysate/extract10 % SDS-PAGEEIF5 antibody dilution: 1:1000.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

EIF5 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EIF5. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-16311 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EIF5 Antibody

  • eIF-5
  • EIF-5A
  • eukaryotic translation initiation factor 5

Background

Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF7558
Species: Hu, Mu, Rt
Applications: WB
NBP1-82648
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-00702
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
H00008894-M09
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC-P
NBP1-85943
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-02669
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-16289
Species: Hu
Applications: WB
NB100-268
Species: Hu, Rt, Po
Applications: WB, IP
NBP1-91994
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-24632
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, IHC-P
NBP2-16293
Species: Hu
Applications: WB, ICC/IF
NBP2-24529
Species: Hu, Mu, Ca, Ch, Pm
Applications: WB, Simple Western, IHC-P
NBP2-38572
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-511
Species: Hu, Mu, Rt
Applications: WB, IP
NBP2-58544
Species: Hu
Applications: WB, ICC/IF
NBP1-91755
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-46580
Species: Hu
Applications: WB, IHC
NBP2-55136
Species: Hu
Applications: ICC/IF
NB100-81898
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-85310
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for EIF5 Antibody (NBP2-16311) (0)

There are no publications for EIF5 Antibody (NBP2-16311).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for EIF5 Antibody (NBP2-16311) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-16311:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot EIF5 NBP2-16311
Enlarge
reviewed by:
Anonymous
WB Human 10/16/2015
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
Lot40401

Blocking

Blocking DetailsBlocker=Skimmed milk, Buffer= 1XTris Buffer Saline+0.1 % Tween 20, 2hrs at RT

Primary Anitbody

Dilution Ratio1:500 over night at 4 degree Celsius

Secondary Antibody

Secondary Descriptionanti-rabbit IgG, HRP
Secondary Manufacturer Cat#7074P2
Secondary Concentration1:20,000

Details

Detection Notes30 seconds exposure

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EIF5 Antibody (NBP2-16311) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional EIF5 Products

Bioinformatics Tool for EIF5 Antibody (NBP2-16311)

Discover related pathways, diseases and genes to EIF5 Antibody (NBP2-16311). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for EIF5 Antibody (NBP2-16311)

Discover more about diseases related to EIF5 Antibody (NBP2-16311).
 

Pathways for EIF5 Antibody (NBP2-16311)

View related products by pathway.

PTMs for EIF5 Antibody (NBP2-16311)

Learn more about PTMs related to EIF5 Antibody (NBP2-16311).

Blogs on EIF5

There are no specific blogs for EIF5, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
10/16/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol EIF5
Uniprot