EIF3F Antibody

Western Blot: EIF3F Antibody [NBP2-16299] - Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: EIF3F Antibody [NBP2-16299] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: EIF3F Antibody [NBP2-16299] - EIF3F antibody detects EIF3F protein at nucleus on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. EIF3F antibody dilution: 1:500.

Immunohistochemistry-Paraffin: EIF3F Antibody [NBP2-16299] - Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using antibody at 1:100 dilution.

• Reactivity: Hu, Mu, Pm
• Applications: WB, ICC/IF, IHC, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

EIF3F Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human EIF3F. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF3F
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Application Notes: Use in chromatin immunoprecipitation reported in scientific literature (PMID: 31527668).
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for EIF3F Antibody

• eIF3 p47
• eIF-3-epsilon
• eIF3-epsilon
• eIF3f
• eIF3-p47
• EIF3S5eukaryotic translation initiation factor 3 subunit F
• Eukaryotic translation initiation factor 3 subunit 5
• eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD)
• eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa
• eukaryotic translation initiation factor 3, subunit F

Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for EIF3F Antibody (NBP2-16299)(1)

Publication using NBP2-16299

• Esteves P, Dard L, Brillac A et al. Nuclear control of lung cancer cells migration, invasion and bioenergetics by eukaryotic translation initiation factor 3F Oncogene 2019-09-19 [PMID: 31527668] (Chemotaxis, Human)

Bioinformatics

• Gene Symbol: EIF3F
• Uniprot:
  • O00303(Human)