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EIF3F Antibody

Images

 
Western Blot: EIF3F Antibody [NBP2-16299] - Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: EIF3F Antibody [NBP2-16299] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: EIF3F Antibody [NBP2-16299] - EIF3F antibody detects EIF3F protein at nucleus on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. EIF3F antibody ...read more
Immunohistochemistry-Paraffin: EIF3F Antibody [NBP2-16299] - Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

EIF3F Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EIF3F. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Chimpanzee (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF3F
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Application Notes
Use in chromatin immunoprecipitation reported in scientific literature (PMID: 31527668).
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-16299 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for EIF3F Antibody

  • eIF3 p47
  • eIF-3-epsilon
  • eIF3-epsilon
  • eIF3f
  • eIF3-p47
  • EIF3S5eukaryotic translation initiation factor 3 subunit F
  • Eukaryotic translation initiation factor 3 subunit 5
  • eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD)
  • eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa
  • eukaryotic translation initiation factor 3, subunit F

Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for EIF3F Antibody (NBP2-16299)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ChIP.


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ChIP
(1)
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Filter By Species
Human
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EIF3F Antibody (NBP2-16299) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for EIF3F Antibody (NBP2-16299)

Discover related pathways, diseases and genes to EIF3F Antibody (NBP2-16299). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Calculators

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Bioinformatics

Gene Symbol EIF3F
Uniprot