EIF3F Antibodies
We offer EIF3F Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each EIF3F Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our EIF3F Antibodies can be used in a variety of model species: Bovine, Chicken, Human, Mouse, Primate, Rat. Use the list below to choose the EIF3F Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our EIF3F polyclonal antibodies.
Alternate Names for EIF3F Antibodies
anti-EIF3F antibody, anti-eIF-3-epsilon antibody, anti-eIF3 p47 antibody, anti-eIF3-epsilon antibody, anti-eIF3-p47 antibody, anti-EIF3S5eukaryotic translation initiation factor 3 subunit F antibody, anti-Eukaryotic translation initiation factor 3 subunit 5 antibody, anti-eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD) antibody, anti-eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa antibody, anti-eukaryotic translation initiation factor 3, subunit F antibody
9 Results for "eif3f" in Primary Antibodies
![Immunohistochemistry: EIF3F Antibody [NBP3-38366] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using EIF3F Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38366_rabbit-eif3f-pab-91220241418188.jpg "Immunohistochemistry: EIF3F Antibody [NBP3-38366] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using EIF3F Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.")
![Western Blot: EIF3F Antibody [NBP3-38366] - Western blot analysis of lysates from HeLa cells, using EIF3F Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38366_rabbit-eif3f-pab-91220241581263.jpg)
![Immunohistochemistry: EIF3F Antibody [NBP3-38366] - Immunohistochemistry analysis of EIF3F in paraffin-embedded Mouse spleen tissue using EIF3F Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38366_rabbit-eif3f-pab-3112025173164.jpg%3Ft=1710215611)
![Immunocytochemistry/ Immunofluorescence: EIF3F Antibody [NBP3-38366] - Immunofluorescence analysis of NIH/3T3 cells using EIF3F Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38366_rabbit-eif3f-pab-3112025173195.jpg%3Ft=1710215611)
![Immunocytochemistry/ Immunofluorescence: EIF3F Antibody [NBP3-38366] - Immunofluorescence analysis of PC-12 cells using EIF3F Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38366_rabbit-eif3f-pab-31120251744423.jpg%3Ft=1710215611)
![Immunohistochemistry: EIF3F Antibody [NBP3-38366] - Immunohistochemistry analysis of EIF3F in paraffin-embedded Human pancreas tissue using EIF3F Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38366_rabbit-eif3f-pab-3112025177024.jpg%3Ft=1710215611)
![Immunohistochemistry: EIF3F Antibody [NBP3-38366] - Immunohistochemistry analysis of EIF3F in paraffin-embedded Human colon tissue using EIF3F Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38366_rabbit-eif3f-pab-31120251704763.jpg%3Ft=1710215611)
![Immunohistochemistry: EIF3F Antibody [NBP3-38366] - Immunohistochemistry analysis of EIF3F in paraffin-embedded Rat brain tissue using EIF3F Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38366_rabbit-eif3f-pab-311202516593614.jpg%3Ft=1710215611)
![Immunocytochemistry/Immunofluorescence: EIF3F Antibody [NBP2-16299] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/EIF3F-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16299-img0003.jpg "Immunocytochemistry/Immunofluorescence: EIF3F Antibody [NBP2-16299] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: EIF3F Antibody [NBP2-16299] - EIF3F antibody detects EIF3F protein at nucleus on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. EIF3F antibody dilution: 1:500.](http://images.novusbio.com/fullsize/EIF3F-Antibody-Immunohistochemistry-Paraffin-NBP2-16299-img0004.jpg)
![Immunohistochemistry-Paraffin: EIF3F Antibody [NBP2-16299] - Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/EIF3F-Antibody-Immunohistochemistry-Paraffin-NBP2-16299-img0002.jpg)
![Western Blot: EIF3F Antibody [NBP2-16299] - Sample (30 ug of whole cell lysate) A: A431 B: HepG2 10% SDS PAGE NBP2-16299 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-16299_rabbit-polyclonal-eif3f-antibody-91020242001022.jpg)
![Western Blot: EIF3F Antibody [NBP2-97760] - Anti-EIF3F rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: NIH3T3 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: COLO205 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 45 kDa](http://images.novusbio.com/fullsize/EIF3F-Antibody-Western-Blot-NBP2-97760-img0006.jpg "Western Blot: EIF3F Antibody [NBP2-97760] - Anti-EIF3F rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: NIH3T3 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: COLO205 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 45 kDa")
![Immunohistochemistry-Paraffin: EIF3F Antibody [NBP2-97760] - Immunochemical staining of human EIF3F in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/EIF3F-Antibody-Immunohistochemistry-Paraffin-NBP2-97760-img0005.jpg)
![Immunohistochemistry-Paraffin: EIF3F Antibody [NBP2-97760] - Immunochemical staining of human EIF3F in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/EIF3F-Antibody-Immunohistochemistry-Paraffin-NBP2-97760-img0004.jpg)
![Immunoprecipitation: EIF3F Antibody [NBP2-97760] - EIF3F was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 ul anti-EIF3F rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-EIF3F rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 45 kDa](http://images.novusbio.com/fullsize/EIF3F-Antibody-Immunoprecipitation-NBP2-97760-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: EIF3F Antibody [NBP2-97760] - Immunofluorescence staining of EIF3F in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97760 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97760_rabbit-polyclonal-eif3f-antibody-235202521451711.png)
![Western Blot: EIF3F Antibody [NBP1-50037] - Detection of Human and Mouse eIF3F by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-eIF3F antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. eIF3F was also immunoprecipitated by rabbit anti-eIF3F antibody BL10962, which recognizes an upstream epitope. For blotting immunoprecipitated eIF3F, the ReliaBLOT (R) Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/EIF3F-Antibody-Western-Blot-NBP1-50037-img0003.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: EIF3F Antibody [NBP1-50037] - Detection of Human and Mouse eIF3F by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-eIF3F antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. eIF3F was also immunoprecipitated by rabbit anti-eIF3F antibody BL10962, which recognizes an upstream epitope. For blotting immunoprecipitated eIF3F, the ReliaBLOT (R) Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: EIF3F Antibody [NBP1-50037] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- eIF3F used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/EIF3F-Antibody-Immunohistochemistry-NBP1-50037-img0006.jpg)
![Western Blot: EIF3F Antibody [NBP2-13952] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/EIF3F-Antibody-Western-Blot-NBP2-13952-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: EIF3F Antibody [NBP2-13952] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: EIF3F Antibody [NBP2-13952] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules.](http://images.novusbio.com/fullsize/EIF3F-Antibody-Immunohistochemistry-Paraffin-NBP2-13952-img0002.jpg)
![Western Blot: EIF3F Antibody [NBP1-77997] - shows detection of endogenous eIF3f in lysates from both control HeLa cells (lane 1) and HeLa cells transformed with the kinase cdk11 (lane 2). Cdk11 is responsible for phosphorylating eIF3f in vivo. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:200. This antibody recognizes both phosphorylated and non-phosphorylated eIF3f.](http://images.novusbio.com/fullsize/EIF3F-Antibody-Western-Blot-NBP1-77997-img0001.jpg "Western Blot: EIF3F Antibody [NBP1-77997] - shows detection of endogenous eIF3f in lysates from both control HeLa cells (lane 1) and HeLa cells transformed with the kinase cdk11 (lane 2). Cdk11 is responsible for phosphorylating eIF3f in vivo. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:200. This antibody recognizes both phosphorylated and non-phosphorylated eIF3f.")
![Immunohistochemistry: EIF3F Antibody [NBP3-12558] - Staining of human EIF3F in human liver with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/EIF3F-Antibody-Immunohistochemistry-NBP3-12558-img0001.jpg "Immunohistochemistry: EIF3F Antibody [NBP3-12558] - Staining of human EIF3F in human liver with rabbit polyclonal antibody at 1:100 dilution.")
![Immunohistochemistry-Paraffin: EIF3F Antibody - BSA Free [NBP3-12558] - Immunochemical staining of human EIF3F in human kidney with NBP3-12558 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-12558_rabbit-polyclonal-eif3f-antibody-235202514492396.png)