RNF139 Antibody (3D10) - Azide and BSA Free

Western Blot: RNF139 Antibody (3D10) [H00011236-M01] - RNF139 monoclonal antibody (M01), clone 3D10 Analysis of RNF139 expression in HepG2.

Immunohistochemistry-Paraffin: RNF139 Antibody (3D10) [H00011236-M01] - Analysis of monoclonal antibody to RNF139 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.

Sandwich ELISA: RNF139 Antibody (3D10) [H00011236-M01] - Detection limit for recombinant GST tagged RNF139 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 3D10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RNF139 Antibody (3D10) - Azide and BSA Free Summary

• Immunogen: RNF139 (NP_009149, 565 a.a. ~ 664 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HYFHALCLRKWLYIQDTCPMCHQKVYIEDDIKDNSNVSNNNGFIPPNETPEEAVREAAAESDRELNEDDSTDCDDDVQRERNGVIQHTGAAAEEFNDDTD
• Specificity: RNF139 - ring finger protein 139
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RNF139
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RNF139 Antibody (3D10) - Azide and BSA Free

• EC 6.3.2.-
• HRCA1
• patched related protein translocated in renal cancer
• RCA1multiple membrane spanning receptor TRC8
• ring finger protein 139MGC31961
• Translocation in renal carcinoma on chromosome 8 protein
• TRC8E3 ubiquitin-protein ligase RNF139

Background

The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNF139 Antibody (H00011236-M01)(1)

Publication using H00011236-M01

• Adams M, Simms RJ, Abdelhamed Z et al. A meckelin-filamin A interaction mediates ciliogenesis. Hum Mol Genet. 2011-12-07 [PMID: 22121117]

Bioinformatics

• Gene Symbol: RNF139
• Entrez:
  • 11236(Human)
• OMIM:
  • 144700(Human)
• Uniprot:
  • NP_009149(Human)