EIF3F Antibody

Western Blot: EIF3F Antibody [NBP1-50037] - Detection of Human and Mouse eIF3F by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-eIF3F antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. eIF3F was also immunoprecipitated by rabbit anti-eIF3F antibody BL10962, which recognizes an upstream epitope. For blotting immunoprecipitated eIF3F, the ReliaBLOT (R) Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Immunohistochemistry-Paraffin: EIF3F Antibody [NBP1-50037] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- eIF3F used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

• Reactivity: Hu, Mu, Pm
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

EIF3F Antibody Summary

• Description: Novus Biologicals Rabbit EIF3F Antibody (NBP1-50037) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 307 and 357 of human Eukaryotic Translation Initiation Factor 3, Subunit F using the numbering given in entry NP_003745.1 (GeneID 8665).
• Predicted Species: Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF3F
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EIF3F Antibody

• eIF3 p47
• eIF-3-epsilon
• eIF3-epsilon
• eIF3f
• eIF3-p47
• EIF3S5eukaryotic translation initiation factor 3 subunit F
• Eukaryotic translation initiation factor 3 subunit 5
• eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD)
• eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa
• eukaryotic translation initiation factor 3, subunit F

Background

eIF3f, also known as eukaryotic translation initiation factor 3 subunit 5, eIF-3 epsilon, and eIF3 p47 subunit, binds to the 40S ribosome and promotes the binding of methionyl-tRNA and mRNA. EIF3f also associates with the complex p170-eIF3. eIF-3 is composed of at least 12 different subunits, eIF3f is one of these subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF3F