EIF3F Antibody

Images

 
Western Blot: EIF3F Antibody [NBP1-50037] - Detection of Human and Mouse eIF3F by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP ...read more
Immunohistochemistry: EIF3F Antibody [NBP1-50037] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- eIF3F used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

EIF3F Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 307 and 357 of human Eukaryotic Translation Initiation Factor 3, Subunit F using the numbering given in entry NP_003745.1 (GeneID 8665).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF3F
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EIF3F Antibody

  • eIF3 p47
  • eIF-3-epsilon
  • eIF3-epsilon
  • eIF3f
  • eIF3-p47
  • EIF3S5eukaryotic translation initiation factor 3 subunit F
  • Eukaryotic translation initiation factor 3 subunit 5
  • eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD)
  • eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa
  • eukaryotic translation initiation factor 3, subunit F

Background

eIF3f, also known as eukaryotic translation initiation factor 3 subunit 5, eIF-3 epsilon, and eIF3 p47 subunit, binds to the 40S ribosome and promotes the binding of methionyl-tRNA and mRNA. EIF3f also associates with the complex p170-eIF3. eIF-3 is composed of at least 12 different subunits, eIF3f is one of these subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EIF3F