eIF3e Antibody

Western Blot: eIF3e Antibody [NBP1-52116] - Analysis of eIF3e in Daudi lysate (35ug protein in RIPA buffer) using NBP1-52116 at 0.03 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

eIF3e Antibody Summary

• Description: Novus Biologicals Goat eIF3e Antibody (NBP1-52116) is a polyclonal antibody validated for use in WB and ELISA. Anti-eIF3e Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KLNQNSRSEAPN corresponding to C-Terminus according to NP_001559.1.
• Epitope: C-KLNQNSRSEAPN
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EIF3E
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in Daudi lysates (calculated MW of 52.2 kDa band according to NP_001559.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for eIF3e Antibody

• eIF-3 p48
• eIF3e
• eIF3-p48
• EIF3S6EIF3-P48
• Eukaryotic translation initiation factor 3 subunit 6
• eukaryotic translation initiation factor 3 subunit E
• eukaryotic translation initiation factor 3, subunit 6 (48kD)
• eukaryotic translation initiation factor 3, subunit 6 48kDa
• eukaryotic translation initiation factor 3, subunit E
• INT6eIF3-p46
• mammary tumor-associated protein INT6
• murine mammary tumor integration site 6 (oncogene homolog)
• Viral integration site protein INT-6 homolog

Background

Int6 is a candidate tumor suppressor in multiple neoplasms, and in particular, breast and lung cancers. The Int6 locus was initially identified as a common insertion site (CIS) in a genetic screen for transforming sequences in a breast cancer mouse model system. Insertion of mouse mammary tumor virus (MMTV) into this locus results in the production of an amino-terminal truncated gene product. Expression of the truncated Int6 product corresponds to cellular transformation in both in vivo and in vitro systems. This gene product plays a role in regulating translation initiation and is a component of the eIF3 translation initiation complex. There is evidence that suggests that Int6 may impart a negative role in the general translational machinery while promoting an increase in the expression of a subset of stressresponsive genes. Taken together, it is of great interest to further study the mechanism by which Int6 is involved in regulating cell growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for eIF3e Antibody (NBP1-52116)(1)

Publication using NBP1-52116

• Zhou M, Sandercock AM, Fraser CS et al. Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3. Proc Natl Acad Sci U S A 2008-11-01 [PMID: 18599441]

Bioinformatics

• Gene Symbol: EIF3E
• Entrez:
  • 3646(Human)
• Uniprot:
  • NP_001559.1(Human)