eIF2B4 Antibody

Images

 
Western Blot: eIF2B4 Antibody [NBP2-16293] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: eIF2B4 Antibody [NBP2-16293] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF2B delta protein diluted at 1:500. Blue: Hoechst 33342 staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.61 mg/ml

Order Details

eIF2B4 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EIF2B delta. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF2B4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (80%), Bovine (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.61 mg/ml
Purity
Immunogen affinity purified

Alternate Names for eIF2B4 Antibody

  • DKFZp586J0119
  • eIF-2B GDP-GTP exchange factor subunit delta
  • EIF2B
  • EIF-2B
  • EIF2BD
  • EIF2Bdelta
  • eukaryotic translation initiation factor 2B, subunit 4 (delta, 67kD)
  • eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa
  • translation initiation factor eIF-2b delta subunit
  • translation initiation factor eIF-2B subunit delta

Background

Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EIF2B4
Uniprot