eIF2A [p Ser51] Antibody (SZ01-06)

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Western Blot: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of Phospho-eIF-2a(S51) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room ...read more
Immunocytochemistry/ Immunofluorescence: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Staining of Hela cells (green) using NBP2-67353. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-eIF2A antibody. The section was pre-treated ...read more
Flow Cytometry: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of Hela cells with Phospho-eIF-2a(S51) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with ...read more
Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human liver tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded human pancreas tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody (SZ01-06) [NBP2-67353] - Analysis of paraffin-embedded mouse placenta tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
SZ01-06
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

eIF2A [p Ser51] Antibody (SZ01-06) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser51 of human eIF-2a. (SwissProt: Q9BY44 Human;SwissProt: Q8BJW6 Mouse;EntrezGene:502531 Rat)
Modification
p Ser51
Localization
Cytoplasmic granule
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
EIF2A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
Publications
Read Publications using
NBP2-67353 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for eIF2A [p Ser51] Antibody (SZ01-06)

  • EIF-2
  • EIF-2A
  • EIF2AEIF2
  • EIF-2alpha
  • eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
  • subunit 1 (alpha, 35kD )

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for eIF2A Antibody (NBP2-67353)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
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Filter By Species
Human
(2)
All Species
Showing Publications 1 - 2 of 2.
Publications using NBP2-67353 Applications Species
Kornicka-Garbowska K, Bourebaba L, ROcken M, Marycz K Sex Hormone Binding Globulin (SHBG) Mitigates ER Stress in Hepatocytes In Vitro and Ex Vivo Cells Mar 30 2021 [PMID: 33808055] (WB, Human) WB Human
Chen Y, Tristan CA, Lu C et al. A Versatile Polypharmacology Platform Promotes Cytoprotection and Viability of Human Pluripotent and Differentiated Cells biorxiv 2019 Oct 22 (WB, Human) WB Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for eIF2A Antibody (NBP2-67353) (0)

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Bioinformatics Tool for eIF2A Antibody (NBP2-67353)

Discover related pathways, diseases and genes to eIF2A Antibody (NBP2-67353). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for eIF2A Antibody (NBP2-67353)

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PTMs for eIF2A Antibody (NBP2-67353)

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Research Areas for eIF2A Antibody (NBP2-67353)

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Blogs on eIF2A.

eIF2alpha - a regulator of global translation in response to cellular stress
Eukaryotic initiation factor 2 (eIF2) regulates global protein translation by binding to Met-tRNA and the 40S ribosome to form the pre-initiation complex. eIF2 is a heterotrimer consisting of alpha, beta, and gamma subunits. The 36kDA eIF2a subuni...  Read full blog post.

PKR - Mediating cellular stress responses through multiple signaling pathways
Protein kinase R (PKR) is an intracellular stress-sensing protein that is able to detect and respond to viral infections. While PKR is able to sense and respond to a variety of signals, dsRNA is a well-characterized ligand. dsRNA produced during v...  Read full blog post.

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Bioinformatics

Gene Symbol EIF2A