eIF2A [p Ser51] Antibody (0011) - Azide and BSA Free

Western Blot: eIF2A [p Ser51] Antibody (0011) [NBP3-23508] - Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (100nM, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using NBP3-23508 at 1:1000 dilution.

• Reactivity: Hu
• Applications: WB
• Clone: 0011
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

eIF2A [p Ser51] Antibody (0011) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Ser51 of human phospho-eIF2A.
• Modification: p Ser51
• Source: HEK293
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EIF2A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for eIF2A [p Ser51] Antibody (0011) - Azide and BSA Free

• EIF-2
• EIF-2A
• EIF2AEIF2
• EIF-2alpha
• eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• subunit 1 (alpha, 35kD )

Background

The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF2A