eIF2 alpha/EIF2S1 Antibody - BSA Free

Western Blot: EIF2S1 Antibody [NBP2-16295] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:2000.

Immunohistochemistry-Paraffin: EIF2S1 Antibody [NBP2-16295] - Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using antibody at 1:100 dilution.

• Reactivity: Hu, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

eIF2 alpha/EIF2S1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human eIF2 alpha/EIF2S1. The exact sequence is proprietary.
• Predicted Species: Xenopus (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF2S1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (94%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for eIF2 alpha/EIF2S1 Antibody - BSA Free

• eIF2 alpha
• EIF2
• EIF-2
• EIF2A
• EIF-2A
• EIF-2alpha
• EIF2S1 eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• EIF2S1

Background

The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: EIF2S1
• Uniprot:
  • P05198()