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HAF109). A specific band was detected for eIF2a at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody." class="big_lightbox" target="_blank">
1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes or 20 mJ/cm2 ultraviolet light (UV) followed by a 30 minute recovery. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Phospho-eIF2a (S52) Monoclonal Antibody (Catalog # MAB39971) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-eIF2a (S52) at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
![Western Blot eIF2 alpha/EIF2S1 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/eIF2_alpha_AF3997_Western_Blot_5377.jpg)
![Western Blot eIF2 alpha/EIF2S1 [p Ser52] Antibody (849159) [Unconjugated]](http://images.novusbio.com/images/antibody/eIF2_alpha_MAB39971_Western_Blot_13024.jpg)
![Simple Western eIF2 alpha/EIF2S1 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/eIF2_alpha_AF3997_Simple_Western_17129.jpg)
![Western Blot eIF2 alpha/EIF2S1 [p Ser52] Antibody (849159) [Unconjugated]](http://images.novusbio.com/fullsize/mab39971_human-phospho-eif2-alpha-s52-mab-clone-849159-western-blot-121220257484419.jpg)
