eIF2 alpha/EIF2S1 [p Ser51] Antibody (E90)

Product Discontinued

eIF2 alpha/EIF2S1 [p Ser51] Antibody (E90) Summary

• Immunogen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human EIF2S1 (phospho S51).
• Modification: p Ser51
• Specificity: The antibody only detects eIF-2a phosphorylated on Serine 51.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EIF2S1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:1000-10000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reacts with: Fruit fly (Drosophila melanogaster), African Green Monkey, Neurospora crassa. Predicted to work with:Bovine, Pig.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 59% PBS, 0.05% BSA and 40% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Protein A or G purified

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for eIF2 alpha/EIF2S1 [p Ser51] Antibody (E90)

• eIF2 alpha
• EIF2
• EIF-2
• EIF2A
• EIF-2A
• EIF-2alpha
• EIF2S1 eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• EIF2S1

Background

EIF2S1, also known as eIF2a, is the alpha subunit of the translation initiation factor eIF2 complex which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. The phosphorylation state of eIF2S1 controls the rate of tRNA translation. When eIF2a is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2S1 is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation. Recombinant human EIF2S1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for eIF2 alpha/EIF2S1 Antibody (NB110-56949)(25)

Publications using NB110-56949

• Farabaugh KT, Krokowski D, Guan BJ et al. PACT-mediated PKR activation acts as a hyperosmotic stress intensity sensor weakening osmoadaptation and enhancing inflammation Elife 2020-03-16 [PMID: 32175843]
• Li SS, Jiang WL, Xiao WQ et al. KMT2D deficiency enhances the anti-cancer activity of L48H37 in pancreatic ductal adenocarcinoma World J Gastrointest Oncol 2019-08-15 [PMID: 31435462]
• Krokowski D, Guan B, Wu J et al. GADD34 function in protein trafficking promotes adaptation to hyperosmotic stress in human corneal cells. Cell Rep 2017-12-05 [PMID: 29212034] (WB, Human)
• Stewart Claire, Estrada Andrea, Kim Paul et al. Regulation of IRE1a by the small molecule inhibitor 4u8c in hepatoma cells. Endoplasmic Reticulum Stress in Diseases 2017-01-01 [PMID: 29098149] (WB, Rat)
• Uppala JK, Gani AR, Ramaiah KVA. Chemical chaperone, TUDCA unlike PBA, mitigates protein aggregation efficiently and resists ER and non-ER stress induced HepG2 cell death. Sci Rep. 2017-06-19 [PMID: 28630443] (FLOW, WB, Human)
• Liu YQ, Jia MQ, Xie ZH et al. Arrestins contribute to amyloid beta-induced cell death via modulation of autophagy and the alpha7nAch receptor in SH-SY5Y cells. Sci Rep. 2017-06-13 [PMID: 28611418] (WB, Human)
• Krokowski D, Jobava R, Guan BJ et al. Coordinated regulation of the neutral amino acid transporter SNAT2 and the protein phosphatase subunit, GADD34, promotes adaptation to increased extracellular osmolarity. J Biol Chem 2015-06-03 [PMID: 26041779]
• Rajesh K, Krishnamoorthy J, Kazimierczak U et al. Phosphorylation of the translation initiation factor eIF2alpha at serine 51 determines the cell fate decisions of Akt in response to oxidative stress Cell Death Dis. 2015-01-16 [PMID: 25590801] (WB, Human)
• Guan B, Krokowski D, Majumder M et al. Translational control during endoplasmic reticulum stress beyond phosphorylation of the translation initiation factor eIF2a. J Biol Chem 2014-05-02 [PMID: 24648524] (WB, Mouse)
• Rouschop KM, Dubois LJ, Keulers TG et al. PERK/eIF2alpha signaling protects therapy resistant hypoxic cells through induction of glutathione synthesis and protection against ROS Proc Natl Acad Sci U S A 2013-03-19 [PMID: 23471998] (IF/IHC, Mouse)
• Sormani R, Delannoy E, Lageix S et al. Sublethal cadmium intoxication in Arabidopsis thaliana impacts translation at multiple levels. Plant Cell Physiol 2011-02-01 [PMID: 21252299] (WB)
• Willis KL, LanglJO, Shisler JL et al. Viral double-stranded RNAs from vaccinia virus early or intermediate gene transcripts possess PKR activating function, resulting in NF-kappaB activation, when the K1 protein is absent or mutated. J Biol Chem 2011-03-01 [PMID: 21183678]
• Xu YX, Liu L, Caffaro CE et al. Inhibition of Golgi apparatus glycosylation causes endoplasmic reticulum stress and decreased protein synthesis. J Biol Chem 2010-08-01 [PMID: 20529871] (WB)
• Lai CW, Aronson DE, Snapp EL et al. BiP availability distinguishes states of homeostasis and stress in the endoplasmic reticulum of living cells. Mol Biol Cell 2010-06-01 [PMID: 20410136] (WB)
• Hernandez CP, Morrow K, Lopez-Barcons LA et al. Pegylated arginase I: a potential therapeutic approach in T-ALL. Blood 2010-06-01 [PMID: 20407034] (WB)
• Szmola R, Sahin-Toth M et al. Pancreatitis-associated chymotrypsinogen C (CTRC) mutant elicits endoplasmic reticulum stress in pancreatic acinar cells. Gut 2010-03-01 [PMID: 19951900] (WB)
• Willis KL, Patel S, Xiang Y et al. The effect of the vaccinia K1 protein on the PKR-eIF2alpha pathway in RK13 and HeLa cells. Virology 2009-11-01 [PMID: 19744687] (WB)
• Wang X, Rao RP, Kosakowska-Cholody T et al. Mitochondrial degeneration and not apoptosis is the primary cause of embryonic lethality in ceramide transfer protein mutant mice. J Cell Biol 2009-01-01 [PMID: 19139267] (WB)
• Lageix S, Lanet E, Pouch-Pelissier MN et al. Arabidopsis eIF2alpha kinase GCN2 is essential for growth in stress conditions and is activated by wounding. BMC Plant Biol 2008-12-01 [PMID: 19108716] (WB)
• Deval C, Talvas J, Chaveroux C et al. Amino-acid limitation induces the GCN2 signaling pathway in myoblasts but not in myotubes. Biochimie 2008-11-01 [PMID: 18706471] (WB)
• Lawson WE, Crossno PF, Polosukhin VV et al. Endoplasmic reticulum stress in alveolar epithelial cells is prominent in IPF: association with altered surfactant protein processing and herpesvirus infection. Am J Physiol Lung Cell Mol Physiol 2008-06-01 [PMID: 18390830] (IF/IHC)
• Goplen N, Gorska MM, Stafford SJ et al. A phosphosite screen identifies autocrine TGF-beta-driven activation of protein kinase R as a survival-limiting factor for eosinophils. J Immunol 2008-03-01 [PMID: 18322238] (ICC/IF)
• Penas C, Guzman MS, Verdu E et al. Spinal cord injury induces endoplasmic reticulum stress with different cell-type dependent response. J Neurochem 2007-08-01 [PMID: 17578450] (WB)
• Fritsch RM, Schneider G, Saur D et al. Translational repression of MCL-1 couples stress-induced eIF2 alpha phosphorylation to mitochondrial apoptosis initiation. J Biol Chem 2007-08-01 [PMID: 17553788] (WB)
• Nemetski SM, Gardner LB. Hypoxic regulation of Id-1 and activation of the unfolded protein response are aberrant in neuroblastoma. J Biol Chem. 2007-01-01 [PMID: 17102133]

Bioinformatics

• Gene Symbol: EIF2S1
• Entrez:
  • 1965(Human)
  • 13665(Mouse)
  • 54318(Rat)
• Uniprot:
  • P05198()