Eg5 Antibody - BSA Free

Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Analysis in human tonsil and pancreas tissues. Corresponding KIF11 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Staining of human tonsil shows moderate to strong cytoplasmic positivity, mainly in germinal center cells.

Western Blot: Eg5 Antibody [NBP1-84887] - Analysis in human cell line A-431.

Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in a subset of glandular cells.

Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Eg5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Eg5 Antibody - BSA Free (NBP1-84887) is a polyclonal antibody validated for use in IHC and WB. Anti-Eg5 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: ALIKEYTEEIERLKRDLAAAREKNGVYISEENFRVMSGKLTVQEEQIVELIEKIGAVEEELNRVTELFMDNKNELDQCKSDLQNKTQELETTQKHLQETKLQLVK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KIF11
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Eg5 Protein (NBP1-84887PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Eg5 Antibody - BSA Free

• Eg5
• HKSP
• KIF11
• kinesin family member 11
• kinesin-like 1
• Kinesin-like protein 1
• kinesin-like protein KIF11
• Kinesin-like spindle protein HKSP
• Kinesin-related motor protein Eg5
• KNSL1
• KNSL1TRIP-5
• thyroid receptor interacting protein 5
• Thyroid receptor-interacting protein 5
• TR-interacting protein 5
• TRIP5
• TRIP-5
• TRIP5EG5

Background

Eg5 is a mitotic motor protein required for chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. Eg-5 is therefore crucial for proper chromosome segregation during cell division. Eg 5 antibodies are useful tools for cell cycle studies and research on mitotic spindles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Eg5 Antibody (NBP1-84887)(1)

Publication using NBP1-84887

• Kuga T, Nie H, Kazami T et al. Lamin B2 prevents chromosome instability by ensuring proper mitotic chromosome segregation. Oncogenesis 2014-03-17 [PMID: 24637494]

Bioinformatics

• Gene Symbol: KIF11