EFHD2 Antibody - BSA Free

Western Blot: EFHD2 Antibody [NBP2-13947] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4

Immunocytochemistry/Immunofluorescence: EFHD2 Antibody [NBP2-13947] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

Immunohistochemistry-Paraffin: EFHD2 Antibody [NBP2-13947] - Staining in human spleen and skeletal muscle tissues using anti-EFHD2 antibody. Corresponding EFHD2 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: EFHD2 Antibody [NBP2-13947] - Staining of human lymph node shows strong cytoplasmic positivity in a subset of non-germinal center cells.

Immunohistochemistry-Paraffin: EFHD2 Antibody [NBP2-13947] - Staining of human skeletal muscle shows low expression as expected.

Immunohistochemistry-Paraffin: EFHD2 Antibody [NBP2-13947] - Staining of human spleen shows high expression.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EFHD2 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: TDELATKLSRRLQMEGEGGGETPEQPGLN
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EFHD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  EFHD2 Protein (NBP2-13947PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (83%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for EFHD2 Antibody - BSA Free

• EF hand domain containing 2
• EF hand domain family, member D2
• EF-hand domain family, member D2
• EF-hand domain-containing protein D2
• MGC4342
• swiprosin 1
• swiprosin-1
• SWS1

Background

Found in lymphocytes; preferentially expressed in CD8+ cells. Contains 2 EF Hand domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EFHD2